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ProteomicsEstablishment of dimethyl labeling-based quantitative acetylproteomics in Arabidopsis
Protein acetylation, one of many types of post-translational modifications (PTMs), is involved in a variety of biological and cellular processes. In the present study, we applied both CsCl density gradient (CDG) centrifugation-based protein fractionation and a dimethyl-labelling-based 4C quantitativ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-03-14 | PXD006177 | Pride
Cscl density gradient (cdg) centrifugation Stable isotope dimethyl labelling Arabidopsis Squa-d Acetylproteomics 4c proteomic workflow Quantitative ptm proteomics 
Metabolomics13C glucose labelling studies using 2D NMR is a useful tool for determining ex vivo whole organ metabolism during hypothermic machine perfusion of kidneys
Background: The aim of this study is to determine the feasibility of using Nuclear Magnetic Resonance (NMR) tracer studies (13C-enriched glucose) to detect ex vivo de novo metabolism in the perfusion fluid and cortical tissue of porcine kidneys during hypothermic machine perfusion (HMP). Methods: Po...
2017-10-16 | MTBLS367 | MetaboLights
ProteomicsQuantitative nascent proteome profiling by pulse labeling of O-propargyl-puromycin and stable isotope labeled amino acids
Recently, a clickable puromycin analog, O-propargyl-puromycin (OPP), was developed and applied to label C-termini of nascent polypeptide chains (NPCs), followed by a proteomic analysis of NPCs that can be affinity purified via click reaction. Despite its advantage, the affinity purification of NPCs ...
ORGANISM(S): Homo Sapiens (human) 
2020-09-17 | PXD019459 | JPOST Repository
ProteomicsTriplex stable isotope dimethyl labelling - Boersema et al.
Not available
ORGANISM(S): Mus musculus (Mouse) Rattus norvegicus (Rat) 
2008-09-23 | PRD000055 | Pride
ProteomicsDimethyl labeling-based quantitative PTM proteomic analysis of drought-regulated phosphoproteomes in soybean drought-tolerant cultivar
The isotopic dimethyl labeling-based quantitative post-translational modification proteomics was applied to study the phosphoproteomic changes associated with the drought responses of two contrasting soybean cultivars. A total of 9,457 non-redundant, unambiguous, label-independent and repeatable pho...
ORGANISM(S): Glycine max 
2023-05-10 | PXD022806 | Pride
Dimethyl labelling Quantitative ptm proteomics Orbitrap fusion lumos Phosphoproteomics Glycine max Overly post-translationally modified protein (opp) Soybean drought-tolerant cultivar 
ProteomicsDimethyl labeling-based quantitative PTM proteomic analysis of drought-regulated phosphoproteomes in soybean drought-tolerant cultivar
The isotopic dimethyl labeling-based quantitative post-translational modification proteomics was applied to study the phosphoproteomic changes associated with the drought responses of two contrasting soybean cultivars. A total of 9,457 non-redundant, unambiguous, label-independent and repeatable pho...
ORGANISM(S): Glycine max 
2021-09-09 | PXD022794 | Pride
Dimethyl labelling Quantitative ptm proteomics Orbitrap fusion lumos Phosphoproteomics Glycine max Overly post-translationally modified protein (opp) Soybean drought-tolerant cultivar 
ProteomicsAn Msp1-containing complex removes orphaned proteins in the mitochondrial outer membrane of trypanosomes (RNAi knockdown data)
The AAA-ATPase Msp1 extracts mislocalized outer membrane proteins and thus contributes to mitochondrial proteostasis. Using pull down experiments, we show that trypanosomal Msp1 localizes to both the glycosome and the mitochondrial outer membrane, where it forms a stable complex with four outer memb...
ORGANISM(S): Trypanosoma brucei 
2023-07-26 | PXD039634 | Pride
Trypanosoma brucei Proteomics Peptide stable isotope dimethyl labeling Mitochondrial quality control Rnai-mediated protein knockdown 
ProteomicsQuantitative global and phosphoproteomic analysis to define the cellular role of AMPK β1-Ser108 phosphorylation
AMP-activated protein kinase (AMPK) is a metabolic stress-sensing enzyme responsible for maintaining cellular energy homeostasis. Activation of AMPK by salicylate and the thienopyridone A-769662 is critically dependent on phosphorylation of Ser108 in the β1 regulatory subunit. We identified the auto...
ORGANISM(S): Mus musculus (Mouse) 
2018-10-24 | PXD006365 | Pride
Autophagy Ulk1 Ampk β1-ser108 
MetabolomicsHyperoxia induces glutamine-fuelled anaplerosis in retinal Müller cells
Premature infants require oxygen supplementation to survive, but excess oxygen causes retinovascular growth suppression that underlies the leading cause of infant blindness known as retinopathy of prematurity (ROP). We analyzed changes in intermediary metabolism during hyperoxia in human retinal end...
2020-03-12 | MTBLS1228 | MetaboLights
ProteomicsCombined phosphoproteome analysis of the response to mycobacterial cord factor reveals a dichotomy of Mincle-dependent and -independent signaling in macrophages
Sensing of Mycobacterium tuberculosis by the immune system relies on recognition by macrophages. Mycobacterial cord factor, trehalose-6,6’-dimycolate (TDM), is the most abundant cell wall glycolipid and binds to the C-type lectin receptor Mincle. To explore the kinase signaling linking the TDM-Mincl...
ORGANISM(S): Mus musculus (Mouse) Mycobacterium tuberculosis 
2019-01-17 | PXD009513 | Pride
Macrophages Mycobacterium tuberculosis Mouse Phosphoprotoemic Dimethyl 
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