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ProteomicsReproducible protein quantitation of 270 human proteins at increased depth using nanoparticle-based fractionation and reaction monitoring mass spectrometry with stable isotope-labelled internal standards
When using a mix of 274 light synthetic peptide standards as surrogates for 270 human plasma proteins, as well as stable isotope-labelled standards as normalizers for targeted quantitative analysis by LC/MRM-MS, the Seer Proteograph allowed for the enrichment and absolute quantitation of up to an ad...
ORGANISM(S): Homo Sapiens 
2025-02-17 | PXD053704 | panorama
UnknownLight contamination in stable isotope-labelled internal peptide standards is frequent and a potential source of false discovery and quantitation error in proteomics.
Not available
S-EPMC8888373 | biostudies-literature
ProteomicsMultiple reaction monitoring assays for large-scale quantitation of proteins from 20 mouse organs and tissues
We developed 7,184 quantitative multiple reaction monitoring mass spectrometry (MRM-MS) assays that measure 2,118 unique proteins in 20 mouse organs and tissues to enable system-level analysis of protein expression in mice. Targets for assay development were selected by untargeted MS analysis, and c...
ORGANISM(S): Mus musculus (Mouse) 
2024-01-03 | PXD021333 | Pride
Mouse tissue proteomics Proteomic phenotyping Mrm assay development Cptac Molecular phenotyping Stable isotope-labelled standards 
MetabolomicsDivergent metabolism between Trypanosoma congolense and Trypanosoma brucei results in differential sensitivity to metabolic inhibition
Animal African Trypanosomiasis (AAT) is a debilitating livestock disease prevalent across sub-Saharan Africa, a main cause of which is the protozoan parasite Trypanosoma congolense. In comparison to the well-studied T. brucei, there is a major paucity of knowledge regarding the biology of T. congole...
2021-07-20 | MTBLS2372 | MetaboLights
MetabolomicsPhotorespiration is linked to DNA methylation by formate as one-carbon source.
DNA methylation is a key epigenetic modification regulating genome organization, stability, and gene expression. Stable DNA methylation critically relies on methyl groups provided through folate-mediated one-carbon (C1) metabolism, yet the origin and regulation of C1 supply remain elusive. Here we d...
2026-01-06 | MTBLS13444 | MetaboLights
ProteomicsSynthetic biology meets proteomics: Construction of à la carte QconCATs for absolute protein quantification
We report a new approach to the assembly and construction of QconCATs, quantitative concatamers for proteomic applications that yield stoichiometric quantities of sets of stable isotope-labelled internal standards. The new approach is based on synthetic biology precepts of biobricks, making use of l...
ORGANISM(S): Escherichia Coli 
2022-07-18 | PXD027047 | panorama
MetabolomicsTargeted polyamine metabolomics of ATP13A4-deficient cellular and mouse models

ATP13A4 is a polyamine transporter enriched in astrocytes that regulates extracellular polyamine homeostasis. This study contains targeted LC-MS/MS metabolomics data from ATP13A4-deficient cellular and mouse models, including C8-D1A astrocytic cells, astrocyte-conditioned medium, mouse cortex, pl...

2026-06-10 | MTBLS14724 | MetaboLights
UnknownStable Isotope Phosphate Labelling of Diverse Metabolites is Enabled by a Family of 18O-Phosphoramidites.
Not available
S-EPMC10947094 | biostudies-literature
UnknownStable Isotope Phosphate Labelling of Diverse Metabolites is Enabled by a Family of 18 O-Phosphoramidites.
Not available
S-EPMC9298905 | biostudies-literature
UnknownConstruction of a la carte QconCAT protein standards for multiplexed quantification of user-specified target proteins.
Not available
S-EPMC8425055 | biostudies-literature
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