We performed endogenous DPPA2/4 immunoprecipitations from whole cell lysates of the human non-small cell lung cancer cell line NCI-H661, followed by LC-MS/MS to identify enriched proteins.
To investigate DPPA2/4 bound proteins on chromatin we performed rapid immunoprecipitation mass spectrometry (RIME) following double crosslinking of chromatin in NCI-H661 non-small cell lung cancer cells clonal cell lines (Wild Type clones c51 & c52, DPPA2 knockout clones c41 & c42, DPPA4 knockout cl...