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MetabolomicsSteady state isotope tracing in C. elegans (GC-MS analyses)

The metabolic network, composed of thousands of metabolic reactions, is essential for any organism. Despite its importance, a systems level understanding of how metabolic network is wired by reactions that carry flux in unperturbed condition and rewired by transcriptionally activating or repressi...

2024-12-03 | MTBLS11742 | MetaboLights
MetabolomicsSteady state isotope tracing in C. elegans (LC-MS analyses)

The metabolic network, composed of thousands of metabolic reactions, is essential for any organism. Despite its importance, a systems level understanding of how metabolic network is wired by reactions that carry flux in unperturbed condition and rewired by transcriptionally activating or repressi...

2024-12-03 | MTBLS11741 | MetaboLights
GenomicsIntegrated genome-wide analysis of transcription factor occupancy, RNA polymerase II binding and steady-state RNA levels identify differentially regulated functional gene classes
Routine methods for assaying steady-state mRNA levels such as RNA-seq and micro-arrays are commonly used as readouts to study the role of transcription factors (TFs) in gene expression regulation. However, cellular RNA levels do not solely depend on activity of TFs and subsequent transcription by RN...
ORGANISM(S): Homo sapiens 
2012-03-09 | E-GEOD-36349 | biostudies-arrayexpress
ProteomicsGlobal analysis of impact of Atg5 mutation on steady-state protein levels
This experiment analyzes the global impact of Atg5-/- mutation on steady-state protein levels by a standard SILAC experiment. WT+vector cells were grown in isotopically labeled media for multiple passages in order to fully label the proteome. Unlabeled Atg5-/- cells and labeled WT+vector cells were ...
ORGANISM(S): Homo sapiens (Human) 
2016-06-14 | PXD003558 | Pride
Steady state protein level Atg5 Human primary fibroblasts 
TranscriptomicsSteady-state hydrogen peroxide induces glycolysis via metabolic reroute in P. aeruginosa and S. aureus
GAPDHs from human pathogens S. aureus and P. aeruginosa can be readily inhibited by ROS-mediated direct oxidation of their catalytic active cysteines. Because of the rapid degradation of H2O2 by bacterial catalase, only steady-state but not one-dose treatment of H2O2 induces rapid metabolic reroute ...
ORGANISM(S): Pseudomonas aeruginosa MPAO1/P1 
2014-05-08 | E-GEOD-55528 | biostudies-arrayexpress
TranscriptomicsSmall Perturbation Approach Reveals Transcriptomic Steady State
The â??small perturbationâ?? approach is critical in studying the â??steady stateâ?? of a biological system. In our experiments, small perturbations were generated by applying a series of repeating intermittent small doses of ultraviolet radiation to a human keratinocyte cell line, HaCaT. The biolog...
ORGANISM(S): Homo sapiens 
2010-06-24 | E-GEOD-7060 | biostudies-arrayexpress
TranscriptomicsChromatin based modeling of transcription rates identifies the contribution of different regulatory layers to steady-state mRNA levels.
Messenger RNA levels in eukaryotes are balanced by two consecutive regulatory layers. Primary, transcriptional regulation at the level of chromatin and secondary, post-transcriptional regulation of the initial transcript in the cytoplasm. Each layer is individually studied in mechanistic detail, whi...
ORGANISM(S): Mus musculus 
2012-09-13 | E-GEOD-34473 | biostudies-arrayexpress
TranscriptomicsChromatin based modeling of transcription rates identifies the contribution of different regulatory layers to steady-state mRNA levels
Messenger RNA levels in eukaryotes are balanced by two consecutive regulatory layers. Primary, transcriptional regulation at the level of chromatin and secondary, post-transcriptional regulation of the initial transcript in the cytoplasm. Each layer is individually studied in mechanistic detail, whi...
ORGANISM(S): Mus musculus 
2012-07-23 | E-GEOD-33252 | biostudies-arrayexpress
TranscriptomicsExpression data from NOD and C57BL/6 mouse pancreas CD8α- Dendritic Cells (DCs) under steady-state and after in-vitro LPS stimulation
Abstract Two major dendritic cell (DC) subsets have been described in the islets of mice: The immunogenic CD8α-CD11b+ DCs and the tolerogenic CD8α+CD103+ DCs. We have recently reported on reduced numbers of the minor population of tolerogenic CD8α+CD103+ DCs in the pancreas of 5 week old pre-dia...
ORGANISM(S): Mus musculus 
2014-07-01 | E-GEOD-45028 | biostudies-arrayexpress
ProteomicsRPE-1 and RPE-1 trisomic AHA p-c and steady state data
We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. In addition, steady state protein levels comparing the RPE- and RPE-1 trisomic cells by standard SILAC labeling...
ORGANISM(S): Homo sapiens (Human) 
2016-10-06 | PXD004915 | Pride
Human Aneuploidy Pre-1 
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