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ProteomicsEscherichia coli sucrose gradient fractionation
We studied localization of E. coli membrane proteome using sucrose gradient fractionation combined with quantitative MS.
ORGANISM(S): Escherichia coli 
2020-02-19 | PXD016403 | Pride
Escherichia coli 
ProteomicsPreparation of tirapazamine-resistant hepatoma cell line
The aim of the project was to obtain murine hepatoma MH22a [1] cell subline, resistant to anticancer agent 3-amino-1,2,4-benzotriazine-1,4-dioxide (tirapazamine, TPZ), and examine the changes of the protein expression as compared with parental cells, with the main emphasis on the changes of redox pr...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-10 | PXD039876 | Pride
Tirapazamine  hepatoma  resistance 
ProteomicsStructural analysis of human ARS2, a platform for co-transcriptional RNA sorting
ARS2 is a highly conserved metazoan protein involved in numerous aspects of nuclear RNA metabolism. As a direct partner of the nuclear cap-binding complex (CBC) it mediates interactions with diverse RNA processing and transport machineries in a transcript-dependent manner. Here we present the human ...
ORGANISM(S): Homo sapiens (Human) 
2018-09-03 | PXD009035 | Pride
Protein interaction Ars2 
ProteomicsThe isoforms of the molecular chaperone Hsp90 from S. cerevisiae differ in structure, function and client range
The molecular chaperone Hsp90 is an important regulator of proteostasis. It has remained unclear why S. cerevisiae possesses two Hsp90 isoforms, the constitutively expressed Hsc82 and the stress-inducible Hsp82. Here, we report distinct differences despite a sequence identity of 97 %. Consistent wit...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-08-12 | PXD013955 | Pride
Yeast Hsp90 Hsc90 
ProteomicsSgt1 interactome analysis under proteostatic stress and steady state conditions
Buildup of misfolded proteins are prevented by chaperone-assisted refolding and/or proteasome dependent degradation. The coordination of these two protein quality control (PQC) systems is not fully understood. We identified the essential Hsp90 co-chaperone Sgt1 as a new member of a general PQC linki...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-07-02 | PXD016174 | Pride
Heat shock Sgt1 Hapi Saccharomyces cerevisiae Protein quality control 
ProteomicsEnhanced interactome capture (eRIC) of steady-state and arsenite stressed HuH7 cell cytoplasmic and nuclear fractions
HuH7 cells were grown under steady-state conditions or exposed 100 µM Na-arsenite for 1h. Subsequently, the cells were irradiated or not with 253 nm UV light (150 mJ/cm^2), processed to nuclear and cytoplasmic fractions, and subjected to eRIC for determining the nuclear and cytoplasmic RNA-binding ...
ORGANISM(S): Homo sapiens (Human) 
2020-04-06 | PXD016735 | Pride
Human Cell culture Arsenite 
ProteomicsEnhanced RNA Interactome capture (eRIC) and RNA Interactome capture (RIC) of Jurkat cells (DSMZ, ACC-282).
1.0 – 1.3 x 10e8 proliferating Jurkat cells at a density of about 1-1.5 x 10e6 cells/mL were employed per sample. Cells were irradiated or not with 254 nm UV light and subjected to eRIC or RIC to determine the RNA-bound proteome. Data correspond to two biologically independent experiments.
ORGANISM(S): Homo sapiens (Human) 
2018-09-03 | PXD010941 | Pride
Ric Eric Rna interactome Rbps Rbpome Rna-binding proteins 
ProteomicsEnhanced RNA Interactome capture (eRIC) and RNA Interactome capture (RIC) of Jurkat cells (DSMZ, ACC-282) exposed to 0.5mM DMOG or vehicle (DMSO) for 6 hours.
1.0 – 1.3 x 10e8 proliferating Jurkat cells at a density of about 1-1.5 x 10e6 cells/mL were employed per sample. Cells were incubated with 0.5mM DMOG (Cayman Chemical Company, 71210) or an equivalent volume of Dimethyl Sulfoxide (DMSO) (vehicle, Merck 1.02950.0500). The DMSO concentration in the...
ORGANISM(S): Homo sapiens (Human) 
2018-09-03 | PXD010942 | Pride
Dmog Ric Eric N6-methyladenosine Epitranscriptome M6a Rna interactome Rbps Rbpome Rna-binding proteins Dimethyloxalylglycine 
ProteomicsIdentification of proteins crosslinked to lysosomal sphingosine and cholesterol probes
HeLa cells were labelled with lysosomally localized photocrosslinkable and clickable probes for sphingosine and cholesterol. Upon photocrosslinking and cell lysis, the protein-lipid complexes were clicked to biotin azide and enriched via Streptavidin-immunoprecipitation. Enriched protein-lipid compl...
ORGANISM(S): Homo sapiens (Human) 
2023-02-07 | PXD039780 | Pride
Lysosomal lipids Photoaffinity labelling Click chemistry 
ProteomicsIncreased levels of the mitochondrial import factor Mia40 can prevent the aggregation of polyQ proteins in the cytosol
The formation of protein aggregates is a hallmark of neurodegenerative diseases. Observations on patient material and model systems demonstrated links between aggregate formation and declining mitochondrial functionality, but the causalities remained unclear. We used yeast as model system to analyze...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-08-05 | PXD023902 | Pride
Huntingtin / mia40 / mitochondria / protein aggregation / protein translocation 
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