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ProteomicsEscherichia coli sucrose gradient fractionation
We studied localization of E. coli membrane proteome using sucrose gradient fractionation combined with quantitative MS.
ORGANISM(S): Escherichia coli 
2020-02-19 | PXD016403 | Pride
Escherichia coli 
ProteomicsPreparation of tirapazamine-resistant hepatoma cell line
The aim of the project was to obtain murine hepatoma MH22a [1] cell subline, resistant to anticancer agent 3-amino-1,2,4-benzotriazine-1,4-dioxide (tirapazamine, TPZ), and examine the changes of the protein expression as compared with parental cells, with the main emphasis on the changes of redox pr...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-10 | PXD039876 | Pride
Tirapazamine  hepatoma  resistance 
ProteomicsThe cardiac phospho-proteome during pressure overload in mice
Trans-aortic constriction (TAC) is a widely used murine model to study pressure overload-induced cardiac hypertrophy and heart failure. Despite its high prevalence during aortic stenosis or chronic arterial hypertension, the global alterations in cardiac proteome and phospho-proteome dynamics follow...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-26 | PXD061784 | Pride
Heart failure Cardiac remodelling Signalling Tac Phosphorylation 
ProteomicsMitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteins
Cellular functionality relies on a well-balanced, but highly dynamic proteome. Dysfunctionof mitochondrial protein import leads to the cytosolic accumulation of mitochondrial precursor proteins which compromise cellular proteostasis and trigger the mitoprotein-induced stress response. To dissect the...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-02-21 | PXD037741 | Pride
Mitochondria / proteasome / protein folding / proteostasis / thermal proteome profiling / stress response 
ProteomicsNematostella extracellular matrix proteome of planula, primary polyp and adult
The aim of this project was to identify all proteins that are part of the extracellular matrix of Nematostella and to investigate the molecular dynamics that occur in different life stages of the complex life cycle with a special focus on the metamorphosing of the planula-polyp transition. Decellula...
ORGANISM(S): Nematostella vectensis 
2025-10-01 | PXD045345 | Pride
Ecm Life stages Matrisome Mesoglea Planula Primary polyp 
ProteomicsStructural analysis of human ARS2, a platform for co-transcriptional RNA sorting
ARS2 is a highly conserved metazoan protein involved in numerous aspects of nuclear RNA metabolism. As a direct partner of the nuclear cap-binding complex (CBC) it mediates interactions with diverse RNA processing and transport machineries in a transcript-dependent manner. Here we present the human ...
ORGANISM(S): Homo sapiens (Human) 
2018-09-03 | PXD009035 | Pride
Protein interaction Ars2 
ProteomicsThe isoforms of the molecular chaperone Hsp90 from S. cerevisiae differ in structure, function and client range
The molecular chaperone Hsp90 is an important regulator of proteostasis. It has remained unclear why S. cerevisiae possesses two Hsp90 isoforms, the constitutively expressed Hsc82 and the stress-inducible Hsp82. Here, we report distinct differences despite a sequence identity of 97 %. Consistent wit...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-08-12 | PXD013955 | Pride
Yeast Hsp90 Hsc90 
ProteomicsProteomic profile of human CHME3 microglia in response to the miR-124-3p modulation in Swedish neuronal cells
We aim to evaluate how the levels of miR-124 in APPSwedish neuronal change the signature of interferon-gamma-stimulated human microglia, in a co-culture system. We want to fing possible pathways involved in cell-to-cell signaling and traficking, as well as inflammation-associated and miRNA targets i...
ORGANISM(S): Homo sapiens (Human) 
2022-06-02 | PXD030315 | Pride
Neuron-microglia crosstalk  mirna intercellular signaling  neuroimmune function  vesicular traficking 
ProteomicsSgt1 interactome analysis under proteostatic stress and steady state conditions
Buildup of misfolded proteins are prevented by chaperone-assisted refolding and/or proteasome dependent degradation. The coordination of these two protein quality control (PQC) systems is not fully understood. We identified the essential Hsp90 co-chaperone Sgt1 as a new member of a general PQC linki...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-07-02 | PXD016174 | Pride
Heat shock Sgt1 Hapi Saccharomyces cerevisiae Protein quality control 
ProteomicsEnhanced interactome capture (eRIC) of steady-state and arsenite stressed HuH7 cell cytoplasmic and nuclear fractions
HuH7 cells were grown under steady-state conditions or exposed 100 µM Na-arsenite for 1h. Subsequently, the cells were irradiated or not with 253 nm UV light (150 mJ/cm^2), processed to nuclear and cytoplasmic fractions, and subjected to eRIC for determining the nuclear and cytoplasmic RNA-binding ...
ORGANISM(S): Homo sapiens (Human) 
2020-04-06 | PXD016735 | Pride
Human Cell culture Arsenite 
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