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TranscriptomicsWidespread Co-translational RNA Decay Reveals Ribosome Dynamics
It is generally assumed that mRNAs undergoing translation are protected from decay. Here, we show that mRNAs are, in fact, co-translationally degraded. This is a widespread and conserved process affecting most genes, where 5′–3′ transcript degradation follows the last translating ribosome, producing...
ORGANISM(S): Schizosaccharomyces pombe 
2015-06-04 | E-GEOD-63120 | biostudies-arrayexpress
TranscriptomicsHeart RNA-Seq of cardiomyocyte-specific and inducible Prmt5 knockout mouse model, AVV9-ACADVL rescue
Our study introduces a novel animal model featuring a cardiomyocyte-specific, inducible knockout of the Prmt5. The primary aim of this model is to investigate the regulatory mechanisms of PRMT5, the main representative of type II PRMTs. We found that PRMT5 controls Acadvl mRNA splicing, and that dis...
ORGANISM(S): Mus musculus 
2026-05-15 | E-MTAB-14396 | biostudies-arrayexpress
TranscriptomicsHeart RNA-Seq of cardiomyocyte-specific and inducible Prmt5 knockout mouse model, nuclear sequencing without treatment
Our study introduces a novel animal model featuring a cardiomyocyte-specific, inducible knockout of the Prmt5. The primary aim of this model is to investigate the regulatory mechanisms of PRMT5, the main representative of type II PRMTs. We found that PRMT5 controls Acadvl mRNA splicing, and that dis...
ORGANISM(S): Mus musculus 
2026-05-15 | E-MTAB-14401 | biostudies-arrayexpress
TranscriptomicsSaccharomyces cerevisiae Transcript Isoform mapping
Extensive transcriptional heterogeneity revealed by isoform profiling Application of TIF-Seq (Transcript IsoForm Sequencing) to S.cerevisiae. The method was applied to simultaneously identify the 5' capped mRNA transcription start site and the 3' polyadenylation site in different conditions: WT cell...
ORGANISM(S): Saccharomyces cerevisiae 
2013-05-06 | E-GEOD-39128 | biostudies-arrayexpress
TranscriptomicsBATSeq of ESC-FCS/ESC-2i/NSC
Single-cell polyadenylation site quantification 48 single cells each of murine embryonic stem cells maintained in FCS+LIF medium ("ESC"), embryonic stem cells maintained in serum free, 2i containing medium ("2i") and neural stem cells ("NSC") were sequenced by BATSeq, a...
ORGANISM(S): Mus musculus 
2015-06-09 | E-GEOD-60768 | biostudies-arrayexpress
TranscriptomicsGenome-wide study of nuclear poly(A) binding protein Nab2p depletion
Polyadenylation of mRNA is a key step in eu- karyotic gene expression. However, despite the ma- jor impact of poly(A) tails on mRNA metabolism, the precise roles of poly(A)-binding proteins (PABPs) in nuclear mRNA biogenesis remain elusive. Here, we demonstrate that rapid nuclear depletion of the S....
ORGANISM(S): Saccharomyces cerevisiae 
2015-08-13 | E-GEOD-65001 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNA Seq of hematopoietic stem and progenitor cells
This dataset is part of a study that investigated how the hematopoietic system coordinates the rapid and efficient regeneration of the megakaryocytic lineage during stress scenarios. We found that the phenotypic hematopoietic stem cell (HSC) compartment contains stem-like megakaryocyte-committed pro...
ORGANISM(S): Mus musculus 
2015-08-31 | E-GEOD-64002 | biostudies-arrayexpress
TranscriptomicsHeart RNA-Seq of cardiomyocyte-specific and inducible Prmt5 knockout mouse model
Our study introduces a novel animal model featuring a cardiomyocyte-specific, inducible knockout of the Prmt5. The primary aim of this model is to investigate the regulatory mechanisms of PRMT5, the main representative of type II PRMTs. We found that PRMT5 controls Acadvl mRNA splicing and that disr...
ORGANISM(S): Mus musculus 
2026-05-15 | E-MTAB-13594 | biostudies-arrayexpress
GenomicsGenome-wide Ste12-binding site mapping in MATa segregants of YJM789 x S96 cross
In this study, we mapped for the first time differences in transcription binding among individuals and elucidated the genetic basis of such variation. Whole-genome Ste12 binding profiles were determined using ChIP-Seq in pheromone-treated cells of 43 segregants of a cross between two highly diverge...
ORGANISM(S): Saccharomyces cerevisiae 
2010-02-18 | E-GEOD-19635 | biostudies-arrayexpress
ProteomicsMislocalization of pathogenic RBM20 variants in dilated cardiomyopathy is caused by loss-of-interaction with Transportin-3
Severe forms of dilated cardiomyopathy (DCM) are associated with point mutations in the alternative splicing regulator RBM20 that are frequently located in the arginine/serine-rich domain (RS-domain). Such mutations can cause defective splicing and cytoplasmic mislocalization, which leads to the for...
ORGANISM(S): Homo sapiens (Human) 
2023-06-19 | PXD038790 | Pride
Human Heart Splicing Dilated cardiomyopathy 
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