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TranscriptomicsRNA Sequencing of default, melanocyte biased and enteric human neural crest populations (NC) and neuroectoderm (CNS)
We have generated expression profiles of three different neural crest populations from human embryonic stem cells. These profiles were compared to a neuroectoderm population. We find that the neural crest populations are separable and distinct. All cell types were differentiated from human embryon...
ORGANISM(S): Homo sapiens 
2016-02-12 | E-GEOD-66148 | biostudies-arrayexpress
ProteomicsTelmisartan is neuroprotective in a hiPSC-derived spinal microtissue model for C9orf72-ALS via inhibition of neuroinflammation
Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease characterized by motor neurons (MN) loss. Mutations in C9orf72 are the leading genetic cause of ALS and involve hexanucleotide repeat expansions (HRE) in the noncoding region of the gene. Neuroinflammation is a key featur...
ORGANISM(S): Homo sapiens (Human) 
2025-07-28 | PXD057067 | Pride
Neuroinflammation  neurodegeneration  amyotrophic lateral sclerosis  c9orf72  human pluripotent stem cells  cellular interactions  motor neurons  astrocytes  microglia  spinal cord  triculture  three-dimension  microtissue  drug screen  sartans  telmisartan 
TranscriptomicsDirected differentiation of placode from human embryonic stem cells
Here we compare the transcriptional profile during the differentiation of hESC into neuroepithelial cells (NSB) (Chambers et. al 2009 Nature Biotechnology) with our new placode differentiation (PIP). Total RNA was isolated at day 1,3, 5 (NSB), 5 (PIP), 7 (NSB), 7 (PIP), 9 (NSB), 9 (PIP), 11 (NSB) an...
ORGANISM(S): Homo sapiens 
2013-12-04 | E-GEOD-51533 | biostudies-arrayexpress
TranscriptomicsHuman iPSC-based Modeling of Late-Onset Disease using Progerin-induced Aging
Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) sets their identity back to an embryonic age. This presents a fundamental hurdle for modeling late-onset disorders using iPSC-derived cells. We therefore developed a strategy to induce age-like features in multiple iPSC-derived li...
ORGANISM(S): Homo sapiens 
2014-01-23 | E-GEOD-52431 | biostudies-arrayexpress
TranscriptomicsSequential stage specific reporter line analysis (SSRLA) of 3 BAC transgenic mESC lines
FACS purified cells from differentiation day 14-15 cells from 3 BAC transgenic mESC lines: Hes::GFP (early), Nurr1::GFP (mid), and Pitx3::YFP (late) DA neuron development reporter lines All three lines were differentiated towards the midbrain dopamine phenotype, and FACS purification was performed ...
ORGANISM(S): Mus musculus 
2012-07-11 | E-GEOD-37446 | biostudies-arrayexpress
TranscriptomicsMolecular and functional characterization of FD-iPSC derived neural crest precursor cells
Global gene expression analysis of FD-iPSC and deribved neural crest cells Here we report the derivation of patient specific Familial Dysautonomia-iPSCs and their directed differentiation into multiple cell types capable of modeling the tissue specific splicing defect in vitro. Undifferentiated hES...
ORGANISM(S): Homo sapiens 
2009-08-31 | E-GEOD-17043 | biostudies-arrayexpress
TranscriptomicsImpaired intrinsic immunity to HSV-1 in human iPSC-derived TLR3-deficient CNS cells
In the course of primary infection with herpes simplex virus 1 (HSV-1), children with inborn errors of the TLR3- and UNC-93B-dependent induction of IFN-α/β and/or IFN-λ immunity are prone to HSV-1 encephalitis (HSE) 1-3. The cells responsible for HSE in these children have yet to be identified. We t...
ORGANISM(S): Homo sapiens 
2012-11-29 | E-GEOD-40593 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human mesenchymal precursor cells derived from embryonic stem cells
Comparisons of expression profiles of human undifferentiated ES cells and Mesenchymal ES cells
ORGANISM(S): Homo sapiens 
2007-08-21 | E-GEOD-2248 | biostudies-arrayexpress
TranscriptomicsmiR-371-3 expression predicts neural differentiation potential in human pluripotent stem cells
The use of pluripotent stem cells in regenerative medicine and disease modeling is complicated by the variation in differentiation properties between lines. In this study, we characterized 13 human embryonic stem cell. (hESC) and 26 human induced pluripotent stem cell (hiPSC) lines to identify mark...
ORGANISM(S): Homo sapiens 
2011-06-01 | E-GEOD-27834 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human embryonic stem cell (hESC) derived mesenchymal precursor cell populations
We have developed efficient protocols for the derivation of mesenchymal precursors from hESCs. While previous protocols were based on mesodermal induction via co-culture of hESCs on OP9 mouse stroma (Barberi et al., PLoS Biology, 2005), our recent work shows the derivation of hESC derived mesenchyma...
ORGANISM(S): Homo sapiens 
2008-06-15 | E-GEOD-7332 | biostudies-arrayexpress
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