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MetabolomicsMultiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment
Membrane lipids and their metabolism have key functions in neurotransmission. Here we provide a quantitative lipid inventory of mouse and rat synaptic junctions. To this end, we developed a multiomics extraction and analysis workflow to probe the interplay of proteins and lipids in synaptic signal t...
2021-09-27 | MTBLS1531 | MetaboLights
ProteomicsEnrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis
Chinese hamster ovary (CHO) cells have been widely employed for production of recombinant proteins (RP) for research and therapeutic purposes. Indeed, this expression system was used for production of most of approved antibodies (84%) in 2015-2018 period. The success of this cell line for RP product...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2020-08-27 | PXD019778 | Pride
Organelles Subcellular fractionation Subcellular proteomics Chinese hamster ovary cells 
ProteomicsBovine sperm plasma membrane proteomics through biotinylation and subcellular enrichment
Mammalian fertilization requires a complex interplay between a sperm cell and an oocyte. A significant proportion of these functions are mediated through the proteomic composition of the sperm surface. These proteomic constituents can present as biomarkers to control and regulate breeding of agricul...
ORGANISM(S): Bos taurus (Bovine) 
2015-03-19 | PXD001096 | Pride
Plasma membrane proteome Reproductive biology Biotinylation Bos taurus Sperm cell 
ProteomicsTMT Screening of Human Samples:SiHa cell
A screen for APOA1 downstream proteins affecting platinum-based chemoresistance using Tandem Mass Tag revealed 64 differentially expressed proteins in SiHa cells, which were subjected to Gene Ontology (annotation, Kyoto Encyclopedia of Genes and Genomes enrichment, subcellular localization, structur...
ORGANISM(S): Cancer 
2022-05-20 | PXD030885 | Pride
Siha cell Tmt screening Human samples 
UnknownSubcellulaRVis: a web-based tool to simplify and visualise subcellular compartment enrichment.
Not available
S-EPMC9252817 | biostudies-literature
ProteomicsSpatial-proteomics reveals phospho-signaling dynamics at subcellular resolution
Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for responding and elicit a coordinated response to stimuli. Mass spectrometry (MS)-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spa...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-11-18 | PXD023690 | Pride
Dia Spatio-temporal dynamics Proteomics Phosphoproteomics 
ProteomicsMass Spectrometry-compatible Subcellular Fractionation
We developed a mass spectrometry-compatible subcellular fractionation protocol. This method involves separating cells into cytoplasmic, organellar, and nuclear fractions without ultracentrifugation.
ORGANISM(S): Homo Sapiens (human) 
2019-11-15 | PXD013581 | JPOST Repository
ProteomicsGlobal Profiling of Protein Lactylation Across Subcellular Fractionation Methods in HCT-116
Protein lactylation is a novel post-translational modification (PTM) involved in many important physiological processes such as macrophage polarization, immune regulation and tumour cell growth. However, most studies to date have focused on the function of lactylation on histones, and little is know...
ORGANISM(S): Homo sapiens (Human) 
2024-12-15 | PXD053474 | Pride
Post-translation modification Subcellular fractionation method Lactylation 
UnknownSubcellular Enrichment Patterns of New Genes in Drosophila Evolution.
Not available
S-EPMC11843443 | biostudies-literature
ProteomicsA click-compatible BmTyr platform for biotin-free proximity labeling and subcellular proteome profiling in primary T cells
Proximity labeling has revolutionized the study of dynamic subcellular proteomes by enabling the capture of transient protein interactions within living cells, yet the application of existing platforms to hard-to-transfect primary cells remains challenging. Here, we leverage a bioorthogonal proximit...
ORGANISM(S): Mus Musculus 
2026-05-09 | PXD078205 |
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