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ProteomicsHPLC-TIMS-TOF-MS of purified mitochondrial I+III2 supercomplex from Arabidopsis thaliana
Mitochondrial I+III2 supercomplex was purified from Arabidopsis thaliana cells using differential centrifugation including Percoll step gradients and continuous sucrose gradients. Supercomplex fractions were digested with trypsin using the SP3 approach. Peptides were further purified with SepPak car...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-12-30 | PXD036482 | Pride
Arabidopsis Supercomplex Mitochondria 
ProteomicsPSI supercomplex copurification using PSI His-tag
Chlamydomonas reinhardtii PSI-Cyt b6f complexes from sucrose density gradient fractions were identified by western blot and SMF spectroscopy, but showed high heterogeneity, as evident from the multiple bands present in the Coomassie stained SDS gel and original EM micrographs. Therefore potential PS...
ORGANISM(S): Chlamydomonas reinhardtii 
2018-10-03 | PXD008857 | Pride
His-tag Psi Supercomplex Chlamydomonas reinhardtii 
ProteomicsImmunoprecipitation of Toxoplasma gondii mETC supercomplex mutant
The apicomplexan parasite Toxoplasma gondii has a divergent mitochondrial electron transport chain (mETC). Complex III and IV associate together to form supercomplexes. We identified and deleted a Complex IV subunit (ApiCox10) which mediates this interaction. We perfomred immunoprecipitation of comp...
ORGANISM(S): Toxoplasma gondii Toxoplasma gondii 
2025-01-15 | PXD053932 | Pride
Toxoplasma Complex iii Metc Supercomplex Mitochondria Complex iv 
ProteomicsAtomic structures of respiratory complex III2 complex IV and supercomplex III2-IV from vascular plants
Mitochondrial complex III (CIII2) and complex IV (CIV) which can associate into a higher-order supercomplex (SC III2 IV) play key roles in respiration. However, structures of these plant complexes remain unknown. We present atomic models of CIII2, CIV and SC III2 IV from Vigna radiata determined by ...
ORGANISM(S): Vigna Radiata (ncbitaxon:157791) 
2020-10-05 | MSV000086237 | MassIVE
UnknownCell Type-Specific Modulation of Respiratory Chain Supercomplex Organization.
Not available
S-EPMC4926459 | biostudies-literature
UnknownIdentification of a protein mediating respiratory supercomplex stability.
Not available
S-EPMC3302151 | biostudies-literature
ProteomicsProteomic analysis in the study of the mitochondrial supercomplex assembly
Mitochondrial supercomplexes are functionally important, but how supercomplexes being assembled is not known. Mitochondrial Complex I has an important role in the assembly of supercomplex. In this study, we generated a series of cell model with shRNA mediated gene silencing or CRISPR-Cas9 gene knock...
ORGANISM(S): Homo Sapiens 
2021-01-08 | PXD023474 |
UnknownMolecular asymmetry of a photosynthetic supercomplex from green sulfur bacteria.
Not available
S-EPMC9529944 | biostudies-literature
UnknownRapid Electron Transfer within the III-IV Supercomplex in Corynebacterium glutamicum.
Not available
S-EPMC5040959 | biostudies-literature
UnknownLifetime imaging of GFP at CoxVIIIa reports respiratory supercomplex assembly in live cells.
Not available
S-EPMC5382582 | biostudies-literature
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