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ProteomicsInter- and Intra-Molecular Interactions of The Arabidopsis Plasma Membrane Proton Pump Revealed Using a Mass Spectrometric-Cleavable Crosslinker
In plants and fungi, the plasma membrane proton pump (H+-ATPase) establishes an electrochemical gradient across the plasma membrane which serves as the driving force for the secondary transport of ions and nutrients across the cell membrane. This is an essential enzyme that functions in many importa...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Saccharomyces cerevisiae (Baker's yeast) 
2023-06-01 | PXD018219 | Pride
Arabidopsis Plasma membrane protein pump Metabolic labeling Dsso Crosslinking 
ProteomicsPhosphoproteomic analysis of osmotic stress response in Arabidopsis
Elucidating how plants sense and respond to water loss is important for identifying genetic and chemical interventions that may help sustain crop yields in water-limiting environments. Currently, the molecular mechanisms involved in the initial perception and response to dehydration are not well und...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-12-19 | PXD001057 | Pride
Phosphorylation arabidopsis stress 
ProteomicsRadiation resistance comparable to natural extremophiles achieved through experimental evolution
Recent human exploitation of high-level sources of ionizing radiation (IR) has generated a corresponding need to understand the effects of IR on living systems. Part of that research has focused on the astonishing capacity of some organisms to survive extraordinary levels of IR exposure. Using exper...
ORGANISM(S): Deinococcus radiodurans Escherichia coli 
2021-09-10 | PXD024784 | Pride
E coli Radiation D radiodurans Oxidation 
ProteomicsThermal proteome profiling of Arabidopsis thaliana
Six biological replicates of Arabidopsis thaliana were subjected to protein extraction, ten-temperature gradient precipitation, TMT labeling, off-line alkaline fractionation, and LC-MS/MS. The resulting data was used downstream for untargeted modeling of protein unfolding and melting temperature est...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-11-23 | PXD011200 | Pride
Lc-ms Arabidopsis Tpp 
ProteomicsIn vivo cross-linking supports a head-to-tail mechanism for regulation of the plant plasma membrane P-type H+-ATPase
In higher plants, a P-type proton pumping ATPase generates the proton-motive force essential for the function of all other transporters and for proper growth and development. X-ray crystallographic studies of the plant plasma membrane proton pump have provided information on amino acids involved in...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-09-24 | PXD010892 | Pride
And arabidopsis thaliana Mass spectrometry Protein cross-linking Protein-protein interaction H+-atpase 
TranscriptomicsProfiling the transcriptome of Thalassiosira pseudonana under silicon replete and deplete growth.
T. pseudonana tiling arrays were used to validate gene models and to predict new genes in the genome of this diatom. The tiling array data validated transcription of about 41% (4,653) of the 11,390 computationally predicted genes. An additional 1,132 transcripts were identified that did not correspo...
ORGANISM(S): Thalassiosira pseudonana CCMP1335 
2010-05-26 | E-GEOD-9661 | biostudies-arrayexpress
TranscriptomicsProfiling the transcriptome of Thalassiosira pseudonana under environmentally relevant growth conditions
T. pseudonana gene-specific arrays were used for differential gene expression. Seven hundred nine genes were differentially expressed by more than 2-fold (Bayesian t-test, p< 0.001) under at least one growth-limiting condition relative to nutrient-replete conditions. A striking result of the hierarc...
ORGANISM(S): Thalassiosira pseudonana CCMP1335 
2010-05-26 | E-GEOD-9660 | biostudies-arrayexpress
ProteomicsMtruncatula - Stem, iBAQ (Marxetal,natbiotechnol2016)
abundance based on iBAQ, Interaction consistency score: 14.8, Coverage: 27
ORGANISM(S): 3880 
2016-00-00 | 2164794630 | PAXDB
ProteomicsMtruncatula - Root, iBAQ (Marxetal,natbiotechnol2016)
abundance based on iBAQ, Interaction consistency score: 13.0, Coverage: 29
ORGANISM(S): 3880 
2016-00-00 | 3047189613 | PAXDB
ProteomicsMtruncatula - Seed, iBAQ (Marxetal,natbiotechnol2016)
abundance based on iBAQ, Interaction consistency score: 13.9, Coverage: 26
ORGANISM(S): 3880 
2016-00-00 | 1575946262 | PAXDB
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