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ProteomicsCD9-HPLC-IAC Isolation of Serum Extracellular Vesicles for Proteomic Analysis
We developed a column-based CD9 antibody-immobilized HPLC immunoaffinity (CD9-HPLC-IAC) technology for EV isolation from a microliter-scale of serum for downstream proteomic analysis. The CD9-HPLC-IAC method achieved EV isolation from 40 μL of serum in 30 min with a yield of 8.0×109 EVs. Proteomic a...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD027025 | Pride
Serum Immunoaffinity isolation Cd9 Extracellular vesicles Hplc Proteomics 
MetabolomicsDeep functional analysis of synII, a 770 kb synthetic yeast chromosome
Here we report the design, construction and characterization of a 770 Kb synthetic yeast chromosome II (synII). With the UPLC-MS method, we demonstrated synII maintains a nearly wild-type phenotype and fitness despite hundreds of designer editing to it. Our phenomics, transcriptomics, proteomics dat...
2016-10-13 | MTBLS296 | MetaboLights
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