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INTRODUCTION: Blackcurrant (Ribes nigrum L.) is an excellent example of a “super fruit” with potential health benefits. Both genotype and cultivation environment are known to affect the chemical composition of blackcurrant, especially ascorbic acid and various phenolic compounds. Environmental condi...
2019-02-12 | MTBLS773 | MetaboLights
Dendrobium species are widely used in traditional Chinese medicine owing to their medicinal and nutritional value, yet the phytochemical diversity and molecular mechanisms underlying theirbioactivities remain poorly understood. To elucidate these traits, we performed an integrative multi-omics study...
2026-06-28 | MTBLS14876 | MetaboLights
Untargeted metabolomics has the potential to improve the predictivity of in vitro toxicity models and therefore may aid the reduction of expensive and laborious animal models. Here we describe a chronic nephrotoxicity study conducted on a human renal proximal tubular epithelial cell line (RPTEC/TERT...
2015-09-07 | MTBLS140 | MetaboLights
Perennial ryegrass (Lolium perenne) is integral to temperate pastoral agriculture, which contributes most of the milk and meat production worldwide. Chemical profiles and diversity of ryegrass offer several opportunities to harness specific traits and elucidate underlying biological mechanism...
2019-03-01 | MTBLS529 | MetaboLights
Salinity and drought stress are the primary cause of crop losses worldwide. In sodic saline soils sodium chloride (NaCl) disrupts normal plant growth and development. The complex interactions of plant systems with abiotic stress have made RNA sequencing a more holistic and appealing approach to stud...
ORGANISM(S): Petunia x hybrida 
The concentrations of twenty kinds of hormones in the follicular fluid were detected by high-performance liquid chromatography–mass spectrometry (HPLC-MS/MS). An Agilent 1200 series high-performance liquid chromatography (HPLC) instrument (Agilent, USA) was utilized. A PAL autosampler (CTC, Swiss) a...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD031559 | Pride
We developed a column-based CD9 antibody-immobilized HPLC immunoaffinity (CD9-HPLC-IAC) technology for EV isolation from a microliter-scale of serum for downstream proteomic analysis. The CD9-HPLC-IAC method achieved EV isolation from 40 μL of serum in 30 min with a yield of 8.0×109 EVs. Proteomic a...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD027025 | Pride
We performed LINP1 RNA pulldown followed by HPLC-MS analysis to identify LINP1-interacting proteins in A431 cells. With high performance liquid chromatography-mass spectrometry (HPLC-MS), the binding protein of LINP1 from RNA pull down was detected by removing non-specific protein which were binding...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD032027 | Pride
We conducted HPLC-MS analysis of the protein mix immunoprecipitated by SIRT7 antibody under non-IR and 8 Gy X-ray irradiation to identify the interacting proteins of SIRT7 in HaCaT cells.
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD033924 | Pride
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