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Proteomics29-Plex Tandem Mass Tag Mass Spectrometry Enabling Accurate Quantification by Interference Correction
Tandem mass tag (TMT) mass spectrometry is a mainstream isobaric chemical labeling strategy for profiling proteomes. Here we present a 29-plex TMT method to combine the 11-plex and 18-plex labeling strategies. The 29-plex method was examined with a pooled sample composed of 1x, 3x and 10x E. coli pe...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD033865 | Pride
Mass spectrometry  liquid chromatography  proteomics  proteome  tandem mass tag  ratio compression  interference  data processing 
ProteomicsExpanding the Landscape of Aging via Orbitrap Astral Mass Spectrometry and Tandem Mass Tag (TMT) Integration
Aging results in progressive decline in physiological function due to the deterioration of essential biological processes, such as transcription and RNA splicing, ultimately increasing mortality risk. Although proteomics is emerging as a crucial tool for elucidating the molecular underpinnings of ag...
ORGANISM(S): Mus musculus (Mouse) 
2025-04-03 | PXD058160 | Pride
Tandem mass tag(tmt) Proteomics Agining Mouse Orbitrap astral mass spectrometer 
ProteomicsExtending the Separation Space with Trapped Ion Mobility Spectrometry Improves the Accuracy of Isobaric Tag-based Quantitation in Proteomic LC/MS/MS
Two-dimensional separation by nano-LC and trapped ion mobility spectrometry (TIMS) prior to Q-TOF tandem mass spectrometry significantly improves the accuracy of isobaric tag-based quantitation in proteome analysis without the need for additional measurement time for TIMS insertion. The obtained pea...
ORGANISM(S): Cellular Organisms 
2020-05-22 | PXD018650 | JPOST Repository
ProteomicsTMTpro: Design and initial evaluation of a novel Proline-based isobaric 16-plex Tandem Mass Tag reagent set
The design and synthesis of a novel proline-reporter-based isobaric Tandem Mass Tag 16 tag set (TMTpro) was carried out and the data uploaded here is a comparison of the performance of the new TMTpro tags with the current commercially available dimethylpiperidine-reporter-based TMT10/11 reagents. Da...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) Rattus norvegicus (Rat) 
2019-11-25 | PXD014750 | Pride
Tmt Tmtpro 
ProteomicsPlasma proteome profiling of patients with in-stent restenosis by tandem mass tag-based quantitative proteomics approach
Analysis of plasma proteins in patients with in-stent restenosis by tandem mass tag-based quantitative proteomics
ORGANISM(S): Homo Sapiens 
2021-06-24 | PXD026890 |
ProteomicsTandem mass tag-based proteomic profiling of drainage fluid
Anastomotic leakage (AL), one of the most severe complications in rectal surgery, is often diagnosed late because of the low specificity of clinical symptoms and limitations of current clinical investigations. Identification of patients with early AL remains challenging. Here, we explored the protei...
ORGANISM(S): Homo Sapiens 
PXD043410 |
UnknownDeep Profiling of Microgram-Scale Proteome by Tandem Mass Tag Mass Spectrometry.
Not available
S-EPMC8262114 | biostudies-literature
UnknownStreamlined Tandem Mass Tag (SL-TMT) Protocol: An Efficient Strategy for Quantitative (Phospho)proteome Profiling Using Tandem Mass Tag-Synchronous Precursor Selection-MS3.
Not available
S-EPMC5994137 | biostudies-literature
ProteomicsIdentification of potential plasma biomarkers for abdominal aortic aneurysm using tandem mass tag quantitative proteomics
Plasma biomarkers that identify abdominal aortic aneurysm (AAA) rupture risk would greatly assist in stratifying patients with small aneurysms. Identification of such biomarkers has hitherto been unsuccessful over a range of studies using different methods. The present study used an alternative prot...
ORGANISM(S): Homo sapiens (Human) 
2018-10-22 | PXD011309 | Pride
Plasma proteomics  immunodepletion  tandem mass tags  tmt  abdominal aortic aneurysm  cardiovascular diseases 
UnknownAutomated Sample Preparation Workflow for Tandem Mass Tag-Based Proteomics.
Not available
S-EPMC10557128 | biostudies-literature
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