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ProteomicsIdentification of ABL Kinase Substrates by Kinase Assay Linked Phosphoproteomics and Multiple Drug Treatments
The kinase assay linked phosphoproteomics (KALIP) strategy was used to identify direct substrates of the Abelson tyrosine kinase (ABL). First, kinase assays using peptides and proteins from chronic myelogenous leukemia cells and recombinant ABL were done to identify kinase substrates in vitro. The A...
ORGANISM(S): Homo Sapiens (human) 
2019-03-20 | PXD005431 | JPOST Repository
ProteomicsProteomics, phosphoproteomics and miRNA analysis of circulating extracellular vesicles through automated and high-throughput isolation
Here, we introduce a magnetic bead-based EV enrichment approach (EVrich) for automated and high-throughput processing of urine samples. Parallel enrichments can be performed in 96-well plates for downstream cargo analysis, including EV characterization, miRNA, proteomics and phosphoproteomics analys...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD033595 | Pride
Mirna Mass spectrometry Prostate cancer Extracellular vesicles Phosphoproteomics 
ProteomicsA pipeline for discovery and verification of glycoproteins from plasma-derived extracellular vesicles as breast cancer biomarkers
Glycoproteins comprise more than half of current FDA-approved protein cancer markers but the development of new glycoproteins as disease biomarkers has been stagnant. Here we present a pipeline to develop glycoproteins from extracellular vesicles (EVs) through integrating quantitative glycoproteomic...
ORGANISM(S): Homo sapiens (Human) 
2018-04-26 | PXD007572 | Pride
Extracellular vesicles Glycoproteomics Biomarker 
ProteomicsIdentification of novel kinases of Tau using fluorescence complementation mass spectrometry (FCMS)
Hyperphosphorylation of the microtubule-associated protein Tau is a major hallmark of Alzheimer’s disease (AD) and other tauopathies. Understanding the protein kinases that phosphorylate Tau is critical for the development of new drugs that target Tau phosphorylation. At present, the repertoire of t...
ORGANISM(S): Homo Sapiens (human) 
2022-11-10 | PXD036956 | JPOST Repository
ProteomicsHigh-throughput Phosphorylation Screening and Validation through Ti(IV)-Nanopolymer Functionalized Reverse Phase PhosphoProtein Array
Protein phosphorylation is one of the most important and widespread molecular regulatory mechanisms that controls almost all aspects of cellular functions in animals and plants. Here, we introduce a novel chemically functionalized Reverse Phase PhosphoProtein Array (RP3A) to capture and measure phos...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-11-12 | PXD010208 | Pride
Phosphorylation 
ProteomicsUniversal plant phosphoproteomics workflow and its application to tomato signaling in response to cold stress
Phosphorylation-mediated signaling transduction plays a crucial role in the regulation of plant defense mechanisms against environmental stresses. To address the high complexity and dynamic range of plant proteomes and phosphoproteomes, we present a universal sample preparation procedure that facili...
ORGANISM(S): Solanum Pimpinellifolium Solanum Lycopersicum 
2018-09-20 | PXD010310 | JPOST Repository
ProteomicsPhosTip for Enrichment of Phosphopeptides from Urinary Extracellular Vesicles
Extracellular vesicles (EVs) are attracting increasing interest with their intrigue role in intercellular communications. Protein phosphorylation in EVs is of great importance for understanding intercellular signaling processes. However, the study of EV phosphoproteomics is impeded by their relative...
ORGANISM(S): Homo sapiens (Human) 
2022-02-15 | PXD020861 | Pride
Phosphopeptides， extracellular vesicles，biomarker 
ProteomicsProteomic Profile of Urinary Extracellular Vesicles Unravelled Biomarkers for Prostate Cancer Recognition
Proteomic profiling of extracellular vesicles (EVs) represents a promising approach for early detection and therapeutic monitoring of diseases such as cancer, which was aimed for identifying novel biomarkers for prostate cancer diagnosis in this study. The focus of this study was to develop a robust...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD037506 | Pride
Extracellular vesicles Prostate cancer Biomarkers 
ProteomicsPhosphoproteins in extracellular vehicles as candidate markers for breast cancer
The state of protein phosphorylation can be a key determinant of cellular physiology such as early stage cancer, but the development of phosphoproteins from biofluids for disease diagnosis remains elusive. Here we demonstrate, for the first time, a strategy to isolate and identify phosphoproteins in...
ORGANISM(S): Homo sapiens (Human) 
2017-03-23 | PXD005214 | Pride
Plasma Extracellular vesicles Phosphoproteomics Biomarker 
ProteomicsPhosphoproteome Analysis of Cerebrospinal Fluid Extracellular Vesicles in Primary Central Nervous System Lymphoma
Primary central nervous system lymphoma(PCNSL) is a rare extra-nodal non-Hodgkin’s lymphoma and accounts for 3%-4% of central nervous system tumors. Recent studies have highlighted the importance of cerebrospinal fluid derived extracellular vesicles in PCNSL. Extracellular vesicles(EVs) are nanoscal...
ORGANISM(S): Homo Sapiens (human) 
2023-07-12 | PXD040744 | JPOST Repository
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