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ProteomicsProtein precipitation of diluted samples with acetone leads to higher protein recovery and reproducibility in comparison with TCA/acetone approach
Proteomic approaches are extremely valuable in many fields of research, where mass spectrometry methods have gained an increasing interest especially due to the ability to perform quantitative analysis. Nonetheless, sample preparation prior to mass spectrometry analysis is of the utmost importance. ...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-04-25 | PXD003653 | Pride
Protein precipitation  acetone  tca/acetone  swath 
UnknownModified TCA/acetone precipitation of plant proteins for proteomic analysis.
Not available
S-EPMC6296544 | biostudies-literature
ProteomicsOptimization of Protein Isolation and Label–free Quantitative Proteomic Analysis in Four Different Tissues of Korean Ginseng
Korean ginseng is one of the most valuable medicinal plants worldwide. Yet, our understanding of ginseng proteomics is largely limited due to difficulties in extraction and resolution of ginseng proteins because of the presence of natural contaminants such as polysaccharides, phenols, and glycosides...
ORGANISM(S): Panax ginseng 
2021-09-07 | PXD022914 | Pride
Label–free proteomics Mva pathway Panax ginseng Ginsenosides  cytochromes p450 Mep pathway Tca/acetone Udp–glycosyltransferase Methanol/chloroform 
UnknownCorrection: Modified TCA/acetone precipitation of plant proteins for proteomic analysis.
Not available
S-EPMC6347226 | biostudies-literature
ProteomicsOptimising sample preparation of the plasma peptidome
We further expanded on previous optimizations to isolate the plasma peptidome and compared various methods to maximize identifications with speed and ease. Previous studies have reported loss of polypeptides binding to high abundant proteins during depletion strategies. We hypothesized that rapid c...
ORGANISM(S): Homo sapiens (Human) 
2017-10-09 | PXD007191 | Pride
Peptidomics Plasma Sample preparation 
Proteomicswheat root MALDI-TOF-MS/MS - Root Proteomics Reveals the Effects of Wood Vinegar on Wheat Growth and Subsequent Tolerance to Drought Stress
To investigate the proteome pattern of wheat roots following a WV seed presoaking treatment under drought stress, comparative proteomic analysis using two-dimensional polyacrylamide gel electrophoresis was employed for wheat roots. Obtained DEPs were digested with trypsin and subjected to 5800 MALDI...
ORGANISM(S): Triticum aestivum (Wheat) 
2019-11-13 | PXD012150 | Pride
Wheat Root Maldi-tof-ms/ms 
UnknownAn Optimized Trichloroacetic Acid/Acetone Precipitation Method for Two-Dimensional Gel Electrophoresis Analysis of Qinchuan Cattle Longissimus Dorsi Muscle Containing High Proportion of Marbling.
Not available
S-EPMC4404140 | biostudies-literature
UnknownOptimization of Protein Isolation and Label-Free Quantitative Proteomic Analysis in Four Different Tissues of Korean Ginseng.
Not available
S-EPMC8309323 | biostudies-literature
ProteomicsMilk bottom-up proteomics: method optimisation.
Milk is a complex fluid whose proteome displays a diverse set of proteins of high abundance such as caseins and medium to low abundance whey proteins such as ß-lactoglobulin, lactoferrin, immunoglobulins, glycoproteins, peptide hormones and enzymes. A sample preparation method that enables high repr...
ORGANISM(S): Bos taurus (Bovine) 
2016-01-26 | PXD002529 | Pride
Replicates. Jersey and holstein cow milk Trypsin digestion Proteome Nlc-esi-ms 
ProteomicsPhosphoproteomics profiling of tobacco mature pollen and pollen activated in vitro
Tobacco mature pollen has extremely desiccated cytoplasm, and is metabolically quiescent. Upon re-hydration it becomes metabolically active and that results in later emergence of rapidly growing pollen tube. These changes in cytoplasm hydration and metabolic activity are accompanied by protein phosp...
ORGANISM(S): Nicotiana tabacum (Common tobacco) Saccharomyces cerevisiae (Baker's yeast) 
2016-01-27 | PXD003042 | Pride
Tabacco Phosphoproteomics Pollen 
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