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TranscriptomicsTranscriptome and translatome profiling of CPX 90m effects in neuroblastoma cells CHP134
Neuroblastoma (NB) is the most frequent extracranial solid tumour of childhood. Clinical courses are highly variable, ranging from spontaneous regression/maturation to rapid progression despite intensive multimodal therapy. The estimation of 5-year event free survival in high-risk patients of only a...
ORGANISM(S): Homo sapiens 
2015-02-04 | E-GEOD-60675 | biostudies-arrayexpress
ProteomicsProteomics of MCF7 cells (LC-MS/MS Orbitrap Fusion Tribrid)
We developed RiboLace, a novel method based on a new puromycin-containing molecule, for the isolation of active ribosomes and associate RNAs by means of an antibody-free and tag-free pull-down approach. We compared riboLace translation estimates with protein levels determined by proteomics in MCF7 c...
ORGANISM(S): Homo sapiens (Human) 
2018-11-21 | PXD009417 | Pride
Mcf7 Polysome profiling Pol-seq Translation Ribolace Rna-seq 
TranscriptomicsTranslatome and transcriptome profiling of estradiol response in MCF7 cells
We subjected MCF7 cells to starvation with 0.5% charcoal treated serum for 48h and then we added 17-beta estradiol (E2) at final concentration of 10 nM, profiling before and after 60 minutes of treatment the transcriptome and the translatome, coming from the polysomal pool of mRNAs after sucrose gra...
ORGANISM(S): Homo sapiens 
2010-12-31 | E-GEOD-22012 | biostudies-arrayexpress
TranscriptomicsTranslatome and transcriptome profiling of EGF response in HeLa cells
Translatome analysis by sucrose gradient centrifugation of cell lysates followed by microarray profiling of the polysomal and subpolysomal RNA fractions represents a way of both studying translational control networks and better approximating the proteomic representation of cells. It is an establish...
ORGANISM(S): Homo sapiens 
2010-05-27 | E-GEOD-20277 | biostudies-arrayexpress
TranscriptomicsTranscriptome profiles of mice intestine and liver upon infection with Salmonella typhimurium (MC71-TT and MC71-DcdtB)
Bacterial genotoxins, produced by several Gram-negative bacteria, induce DNA damage in the target cells. While the responses induced in the host cells have been extensively studied in vitro, the role of the genotoxins as effectors during the course of acute and chronic infections remains poorly char...
ORGANISM(S): Mus musculus 
2016-03-31 | E-GEOD-72046 | biostudies-arrayexpress
TranscriptomicsTranscriptome and translatome profiling of MCF7 vector cells after p53 activation
Several genome-wide transcriptome analyses that focused on p53-induced cellular responses in many cellular contexts have continued to expand the already vast p53-regulated transcriptional networks. To investigate post-transcriptional controls as an additional dimension of p53-directed gene expressio...
ORGANISM(S): Homo sapiens 
2014-04-17 | E-GEOD-50650 | biostudies-arrayexpress
TranscriptomicsCrossroads of the p53, ER, NFkB stress response networks
We explored the combinatorial interactions between p53, estrogen receptors and NFkB using the breast adenocarcinoma-derived MCF7 cells. Recently, we have established that p53 can modulate transcription also through non-canonical response elements (REs), consisting of half-sites and ¾ sites. In parti...
ORGANISM(S): Homo sapiens 
2011-06-01 | E-GEOD-24065 | biostudies-arrayexpress
TranscriptomicsTranscriptome and translatome profiling of CPX 24h effects in neuroblastoma cells CHP134
Neuroblastoma (NB) is the most frequent extracranial solid tumour of childhood. Clinical courses are highly variable, ranging from spontaneous regression/maturation to rapid progression despite intensive multimodal therapy. The estimation of 5-year event free survival in high-risk patients of only a...
ORGANISM(S): Homo sapiens 
2016-06-01 | E-GEOD-53187 | biostudies-arrayexpress
TranscriptomicsTranslatome and transcriptome profiling of SH-SY5Y cells treated with sublytic doses of staphylococcal alpha-hemolysin
Genome-wide analysis of translation has the potential to provide major contributions in understanding the pathophysiology of infection processes, given the complex interplay between pathogens and host cells. Informations about the translational state of mRNAs or the activity of RNA binding proteins ...
ORGANISM(S): Homo sapiens 
2015-04-01 | E-GEOD-50652 | biostudies-arrayexpress
TranscriptomicsTranslatome and transcriptome profiling of FK866 response in Jurkat cells
We treated Jurkat cells for 48 hr with a sublethal dose of FK866 (5 nM) and DMSO (Mock, control treatment). RNA samples for microarrays derived from fractionated samples by sucrose gradient (sub-polysomes, polysomes), giving us the chance to perform an analysis among polysomal/subpolysomal distribu...
ORGANISM(S): Homo sapiens 
2015-04-01 | E-GEOD-53772 | biostudies-arrayexpress
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