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Paired-end sequencing study of nucleosomes and immuno-precipitated Tfc1 and Brf1 complexes from MNase-digested nuclei. Nucleosomal DNA, input DNA and DNA from immunopurified TFIIIB and TFIIIC complexes (IP) were subjected to paired-end sequencing.
ORGANISM(S): Saccharomyces cerevisiae 
TFIIIB roadblocks control alternative cleavage and polyadenylation site selection
To test the functional impact of Pol III on controlling alternative CPA site selection, we depleted, Rna15p, a component of the cleavage and polyadenylation factor I, Bdp1p, a core component of TFIIIB, and Bdp1p/Rna15p together, from the nucleus and assessed the effect on 3´ UTR lengths of genes nea...
ORGANISM(S): Saccharomyces cerevisiae 
2025-12-02 | GSE311276 | GEO
ChAHP Silences SINE Retrotransposons by Inhibiting TFIIIB Recruitment [RNA-seq]
ChAHP Silences SINE Retrotransposons by Inhibiting TFIIIB Recruitment [ChIP-seq]
Epigenetic control is an important aspect of gene regulation. Despite detailed understanding of many examples, the transcription of non-coding RNA genes by RNA polymerase (pol) III is less well characterized. Here we profile the epigenetic features of pol III target genes throughout the human gen...
ORGANISM(S): Homo sapiens 
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