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ProteomicsComparative proteomics of reduced protein phosphatase inhibitor-2 expression in the loss of function mutant Arabidopsis
Protein phosphatase inhibitor-2 (PPI-2) is a conserved eukaryotic effector protein that inhibits type one protein phosphatases (TOPP). A transfer-DNA knockdown of AtPPI-2 resulted in stunted growth in both vegetative and reproductive phases of Arabidopsis development. At the cellular level, AtPPI-2 ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-12-14 | PXD006568 | Pride
Arabidopsis Comparative proteomics Type-1 phosphatase Protein-protein interaction Ppi-2 Ribosome biogenesis Phosphatase inhibitor 
ProteomicsUnveiling orphan receptor-like kinases in plants: novel client discovery using high-confidence library predictions in the Kinase–Client (KiC) assay
Plants are remarkable in their ability to adapt to changing environments, with receptor-like kinases (RLKs) playing a pivotal role in perceiving and transmitting environmental cues into cellular responses. Despite extensive research on RLKs from the plant kingdom, the function and activity (i.e., th...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-03-25 | PXD047713 | Pride
Phosphopeptides P2k1 In vitro screening Protein kinase Deep learning 
ProteomicsIdentifying receptor kinase substrates using an 8,000 peptide kinase client library enriched for conserved phosphorylation sites
In eukaryotic organisms, protein kinases regulate diverse protein activities and signaling pathways through phosphorylation of specific protein substrates. Isolating and characterizing kinase substrates is vital for defining downstream signaling pathways. The Kinase Client (KiC) assay is an in vitro...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-02-26 | PXD056072 | Pride
Candidates P2k1 Phosphopeptide 
ProteomicsAnalysis of protein phosphorylation in response to the ATP treatment in Arabidopsis thaliana
The project intended to reveal protein phosphorylation patterns in Arabidopsis thaliana in response to ATP. For this purpose, Arabidopsis thaliana plants, including WT, ATP receptor mutants (p2k1, p2k2, and double mutant p2k1/p2k2), and P2K1 overexpression plants, were treated with ATP or buffer (as...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
MSV000095888 | MassIVE
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