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ProteomicsThermophilic Geobacillus WSUCF1 secretome for saccharification of AFEX and EA pretreated corn stover
It is difficult to identify individual hydrolyzing enzymes using routine enzyme assays in the laboratory, which only give a collective picture of the secretome enzymatic activity. A proteomic analysis method based on liquid chromatography followed by tandem mass spectrometry (LCMS/MS) has proven sig...
ORGANISM(S): Geobacillus sp. WSUCF1 
2022-08-12 | PXD031733 | Pride
Geobacillus Thermofisher ltq-ft ultra mass spectrometer Lc-msms 
ProteomicsGeLC-MS/MS analysis of hen egg vitelline membrane from freshly-laid eggs and eggs stored for 28 days at 4°C and 20°C
The hen egg vitelline membrane is a protein layer separating the yolk content from the egg white. The integrity of egg vitelline membrane is important for both the shell egg and the egg product industries. However, its physical properties are altered during prolonged storage, depending on storage te...
ORGANISM(S): Gallus gallus (Chicken) 
2026-03-16 | PXD066675 | Pride
Sds-page Egg Vitelline membrane Chicken Nanolc-ms/ms Storage 
ProteomicsMapping the Interaction between Eukaryotic Initiation Factor 4A (eIF4A) and the Inhibitor Hippuristanol Using Carbene Footprinting and Mass Spectrometry
Protein-ligand interactions are central to protein activity and cell functionality. Improved knowledge of these relationships greatly benefits our understanding of key biological processes and aids in rational drug design towards the treatment of clinically relevant diseases. Carbene footprinting is...
ORGANISM(S): Homo sapiens (Human) 
2021-08-25 | PXD025105 | Pride
Protein footprinting Mapping ligand binding Carbene labelling 
ProteomicsDrosophila immune response - Landscape of protein-protein interactions in Drosophila immune deficiency signaling during bacterial challenge
This project aims to define, by using a functional proteomic approach, the molecular partners associated with the canonical coupound of the IMD pathway. The Drosophila defense against pathogens largely relies on the activation of two major pathways, IMD and TOLL. The IMD pathway is activated mainly ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2013-05-22 | PXD000129 | Pride
Drosophila Imd Lcms/ms 
MetabolomicsUntargeted extraction of metabolites 13C labeling profiles from time course labeling switch experiment
Dynamic isotope labeling data provide crucial information about the operation of metabolic pathways, and are commonly generated via liquid chromatography mass spectrometry (LC-MS). Metabolome-wide analysis is challenging as it requires grouping of metabolite features over different samples. We devel...
2015-10-19 | MTBLS228 | MetaboLights
MetabolomicsUntargeted extraction of metabolites 13C labeling profiles from time course labeling switch experiment
Dynamic isotope labeling data provide crucial information about the operation of metabolic pathways, and are commonly generated via liquid chromatography mass spectrometry (LC-MS). Metabolome-wide analysis is challenging as it requires grouping of metabolite features over different samples. We devel...
2015-10-19 | MTBLS229 | MetaboLights
ProteomicsTruncated Tau - Role of the Tau N-terminal region in microtubule stabilization revealed by new endogenous truncated forms
Tau is a microtubule-associated protein that ensures neuronal shape and function. Besides, Tau is a central player in Alzheimer’s disease (AD) and related Tauopathies where it is found aggregated in degenerating neurons. Mechanisms leading to Tau pathology and its progression are far from being eluc...
ORGANISM(S): Homo sapiens (Human) 
2015-05-15 | PXD001353 | Pride
Tau  alzheimer's disease  human  lc ms/ms 
ProteomicsMSP22.8 is a protease inhibitor-like protein involved in shell mineralization in the edible mussel Mytilus galloprovincialis
MSP22.8 is a shell matrix protein described in Mytilus galloprovincialis. During the characterization of the monoclonal antibody M22.8, we have demostrated that MSP22.8 is secreted into the extrapallial space by cells of the mantle edge epithelium. The protein is detected in the extrapallial fluid a...
ORGANISM(S): Mytilus galloprovincialis (Mediterranean mussel) 
2017-08-15 | PXD004706 | Pride
Lc-msms Extrapallial liquid Shell matrix protein Mytilus galloprovincialis 
ProteomicsHierarchical regulation of oxygen-sensing through coupled transducers in Arabidopsis thaliana
The sensing of molecular oxygen is an essential component of development and environmental response in both animals and plants. Few substrates of the plant oxygen-sensing PLANT CYSTEINE OXIDASE (PCO) N-degron pathway have been identified. Here we show that HYPOXIA TRANSDUCER1 (HYT1, previously named...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-09-21 | PXD083373 | Pride
Arabidopsis thaliana hyt1 lc-ms data 
ProteomicsProteomics of Abiotrophia defectiva protein secretion
This projects comprises of proteomic analysis of extracellular proteins secreted by A. defectiva, an oral bacterium occassioanlly causing infective endocarditis.
ORGANISM(S): Abiotrophia defectiva ATCC 49176 
2024-05-21 | PXD015631 | Pride
A. defectiva Proteomics Oral Endocarditis Secretome 
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