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Proteomics‘Shotgun’ Proteome Analyses Do Not Require Alkylation of Cysteine
It is a common belief that reduction of disulfide bridges and alkylation of thiols in proteins are indispensable steps in proteomic sample preparation. Since this chemical procedure is often incomplete and prone to side reactions and can results in various artefacts we reexamined its importance. We ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-01-21 | PXD014000 | Pride
Thiol alkylation Iodoacetamide Shotgun proteomics 
TranscriptomicsThe global expression profile of Bacillus subtilis to the toxic carbonyls methylglyoxal (MG) and formaldehyde (FA)
Abstract of associated menuscript; Quinones and alpha,beta-unsaturated carbonyls are naturally occurring electrophiles that target cysteine residues via thiol-(S)-alkylation. We analyzed the global expression profile of Bacillus subtilis to the toxic carbonyls methylglyoxal (MG) and formaldehyde (FA...
ORGANISM(S): Bacillus subtilis 
2010-05-15 | E-GEOD-13925 | biostudies-arrayexpress
GenomicsThiol-linked alkylation for the metabolic sequencing of RNA
Thiol-linked alkylation for the metabolic sequencing of RNA
PRJNA390259 | ENA
TranscriptomicsSlamSeq of subcellular compartments in mouse primary cortical neurons
We performed SlamSeq (thiol(SH)-linked alkylation for metabolic sequencing) to estimate mRNA half-lives in subcellular compartments (neurites, soma-cytoplasm and nucleus) of primary cortical neurons.
ORGANISM(S): Mus musculus 
2023-07-12 | E-MTAB-12888 | biostudies-arrayexpress
OtherThiol-linked alkylation for the metabolic sequencing of RNA [Primer Extension Assays]
Gene expression profiling by high-throughput sequencing reveals qualitative and quantitative changes in RNA species at steady-state but obscures the intracellular dynamics of RNA transcription, processing and decay. We developed thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM-s...
ORGANISM(S): synthetic construct 
2017-09-23 | GSE99969 | GEO
ProteomicsDifferential alkylation of PHLPP2
To determine the accessibility of cysteines in purified PHLPP2, we assessed their reactivity with iodoacetamide under native conditions. After denaturing with urea and reducing potential disulfide bridges with TCEP, the remaining free cysteines were modified with methyl methanethiosulfonate. This 2-...
ORGANISM(S): Homo sapiens (Human) 
2025-03-13 | PXD052551 | Pride
Differential alkylation Free thiols 
TranscriptomicsThiol-linked alkylation for the metabolic sequencing of RNA [Transcriptional inhibition by Actinomycin D]
Gene expression profiling by high-throughput sequencing reveals qualitative and quantitative changes in RNA species at steady-state but obscures the intracellular dynamics of RNA transcription, processing and decay. We developed thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM-s...
ORGANISM(S): Mus musculus 
2017-09-23 | GSE99975 | GEO
ProteomicsAnalysis of ageing-dependent thiol oxidation reveals early oxidation of proteins involved in core proteostasis functions
Oxidants have a profound impact on biological systems in physiology and under pathological conditions. Oxidative post-translational modifications of protein thiols are well-recognized as a readily occurring alteration of proteins. Changes in protein thiol redox state can modify the function of prote...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-02-07 | PXD042047 | Pride
Yeast Oxicat Proteostasis Thiol oxidation 
UnknownThiol-linked alkylation of RNA to assess expression dynamics.
Not available
S-EPMC5712218 | biostudies-literature
TranscriptomicsThiol-linked alkylation for the metabolic sequencing of RNA [SLAM-seq in wildtype and Mettl3 knockout mES cells]
Gene expression profiling by high-throughput sequencing reveals qualitative and quantitative changes in RNA species at steady-state but obscures the intracellular dynamics of RNA transcription, processing and decay. We developed thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM-s...
ORGANISM(S): Mus musculus 
2017-09-23 | GSE99974 | GEO
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