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ProteomicsOne-STAGE Tip Method for TMT based Proteomic Analysis of Minimal Amount of Cells
LC-MS-based profiling of proteomes with isobaric tag labeling from low-quantity biological and clinical samples, including needle-core biopsies and laser capture microdissection, has been challenging due to limited amount and sample loss during preparation. To address this problem, we developed OnM ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-04-18 | MSV000091734 | MassIVE
ProteomicsReverse-phase liquid chromatography (RPLC) in Combination with Tip-Based Strong Cation Exchange (SCX-Tip) Fractionation, ReST, an Alternative Approach for Cross-linked Peptides analysis
Chemical cross-linking in combination with mass spectrometry (XL-MS) has emerged as a useful method for structural elucidation of proteins and protein complexes. Efficient enrichment procedures are necessary to analyze cross-linked products due to their relatively low abundance. Currently, strong ca...
ORGANISM(S): Homo sapiens (Human) 
2022-06-16 | PXD023817 | Pride
Scx-tip Mass spectrometry Fractionation Chemical cross-linking 
TranscriptomicsmiR-221 is required for endothelial tip cell behaviors during vascular development
Through deep sequencing and functional screening in zebrafish, we find that miR-221 is essential for angiogenesis. miR-221 knockdown phenocopied defects associated with loss of the tip cell-expressed Flt4 receptor. Furthermore, miR-221 was required for tip cell proliferation and migration, as well ...
ORGANISM(S): Danio rerio 
2012-02-28 | E-GEOD-35078 | biostudies-arrayexpress
GenomicsTranscriptome gene expression analysis of root tip, leaf tip, shoot tip, root (without root tip) and leaf (without leaf tip) from Ceratopteris richardii sporophytes
Transcriptome gene expression analysis of root tip, leaf tip, shoot tip, root (without root tip) and leaf (without leaf tip) from Ceratopteris richardii sporophytes
PRJNA578676 | ENA
TranscriptomicsEndothelin2 signaling in the neural retina promotes the endothelial tip cell state and inhibits angiogenesis
Endothelin signaling is required for neural crest migration and homeostatic regulation of blood pressure. Here we report that constitutive over-expression of Endothelin-2 (Edn2) in the mouse retina perturbs vascular development by inhibiting endothelial cell (EC) migration across the retinal surfac...
ORGANISM(S): Mus musculus 
2014-01-01 | E-GEOD-50059 | biostudies-arrayexpress
ProteomicsA streamlined tandem tip-based workflow for sensitive nanoscale phosphoproteomics
Effective nanoscale phosphoproteome analysis remains a daunting task, primarily due to significant sample loss associated with non-specific surface adsorption during phosphopeptide enrichment and other sample processing steps. To address this issue, we have developed a tandem tip-based C18-IMAC-C18 ...
ORGANISM(S): Homo Sapiens (human) 
2023-01-27 | PXD032019 | JPOST Repository
TranscriptomicsRNA-Seq of sika deer antler-tip during the ossification stage
To obtain an overview of the antler tip gene expression profile during rapid growth period, a cDNA sample was prepared from antler tip and sequenced using the Illumina sequencing platform.
ORGANISM(S): Cervus nippon 
2011-10-25 | E-MTAB-802 | biostudies-arrayexpress
ProteomicsOne-step N-terminomics based on isolation of protein N-terminal peptides from LysargiNase digests by tip-based strong cation exchange chromatography
Large-scale isolation of protein N-terminal peptides from LysargiNase digests was achieved by one-step strong cation exchange chromatography with disposable and parallel-processable pipette-tip columns. Novel cleavage sites for signal or transit peptides and non-canonical translation initiation site...
ORGANISM(S): Homo Sapiens (human) 
2024-08-13 | PXD048917 | JPOST Repository
TranscriptomicsThe Transposon Insertion site Profiling Chip (TIP-chip)
We have developed a microarray intended for use in finding all transposons in a region of interest. By selectively amplifying and hybridizing transposon flanking DNA to our array, we can localize all transposons in the region present on our TIP-chip, a dense tiling array. We have tested our technolo...
ORGANISM(S): Saccharomyces cerevisiae 
2010-07-01 | E-GEOD-5646 | biostudies-arrayexpress
TranscriptomicsRNA-seq comparison of fetal lung and cultured fetal lung derived organoids with pluripotent stem cell derived lung organoids
Underdeveloped lungs are the primary cause of death in premature infants, however, little is known about stem and progenitor cell maintenance during human lung development. In this study, we have identified that FGF7, Retinoic Acid and CHIR-99021, a small molecule that inhibits GSK3 to activate Wnt ...
ORGANISM(S): Homo sapiens 
2018-11-17 | E-MTAB-6023 | biostudies-arrayexpress
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