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MetabolomicsTissue metabolites in diffuse glioma and their modulations by IDH1 mutation, histology, and treatment
The discovery of the oncometabolite 2-hydroxyglutarate in isocitrate dehydrogenase 1-mutated (IDH1-mutated) tumor entities affirmed the role of metabolism in cancer. However, large databases with tissue metabolites that are modulated by IDH1 mutation remain an area of development. Here, we present a...
2022-09-07 | MTBLS3873 | MetaboLights
MetabolomicsMetabolomic alterations in invasive ductal carcinoma of breast: A comprehensive metabolomic study using tissue and serum samples
Invasive ductal carcinoma (IDC) is the most common type of breast cancer and the leading cause of breast cancer related mortality. In the present study, metabolomic profiles of 72 tissue samples and 146 serum samples were analysed using targeted liquid chromatography multiple reaction monitoring mas...
2018-10-16 | MTBLS552 | MetaboLights
ProteomicsHomogenization of tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization
Tissue samples from human tonsils and rat pancreas were subjected to a novel laser-based homogenization method, the PIRL-DIVE homogenization, and to a conventional mechanical tissue homogenization procedure (bead mill and a cryo-grinder). The protein species composition of the protein extracts were ...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2016-01-25 | PXD003434 | Pride
Protein species Sample preparation Proteolysis Pirl Tissue homogenization Lc-msms Pirl-dive 
MetabolomicsUntargeted gas chromatography–mass spectrometry-based metabolomics analysis of kidney and liver tissue from the Lewis Polycystic Kidney rat
Polycystic kidney disease (PKD) encompasses a spectrum of inherited disorders that lead to end-stage renal disease (ESRD). There is no cure for PKD and current treatment options are limited to renal replacement therapy and transplantation. A better understanding of the pathobiology of PKD is needed ...
2023-01-26 | MTBLS748 | MetaboLights
ProteomicsSampling and homogenization of nanoliter tissue with a nanosecond infrared laser (NIRL) for mass spectrometric proteomics
It was recently shown that ultrashort pulse infrared (IR) lasers, operating at the wavelength of the OH vibration stretching band of water, are highly efficient at softly sampling and homogenizing biological tissue. We utilized a nanosecond infrared laser (NIRL) for tissue sampling and homogenizatio...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-17 | PXD027700 | Pride
Laser ablation Tissue sampling  nanosecond infrared laser Proteomics  mass spectrometry 
MetabolomicsGSDME-controlled pyroptosis promotes muscle repair by coordinating tissue resident macrophages and fibro-adipogenic progenitors
GSDME (Gasdermin E) is a critical protein known for its role in pyroptosis, a form of programmed cell death associated with inflammation. We demonstrated that GSDME as a crucial mediator in muscle repair, particularly in the context of muscle damage induced by cardiotoxin (CTX). Specifically, we fou...
2026-01-05 | MTBLS10509 | MetaboLights
ProteomicsA Standard Operating Procedure for Protein Extraction from Abdominal Aortic Aneurysm Tissue: Enhancing Proteomics Applications
The identification of putative biomarkers for predicting abdominal aortic aneurysm (AAA) progression can be achieved through proteomic strategies, particularly with shotgun proteomics. However, the heterogeneity of AAA tissue poses challenges for tissue homogenization and protein extraction, comprom...
ORGANISM(S): Homo sapiens (Human) 
2026-01-05 | PXD060417 | Pride
Abdominal aortic aneurysm  aortic tissue homogenization  protein extraction  proteomics  sop 
UnknownImpact of soft tissue homogenization methods on RNA quality.
Not available
S-EPMC12031802 | biostudies-literature
UnknownOptimization of lung tissue pre-treatment by bead homogenization for subsequent culturomics.
Not available
S-EPMC11442450 | biostudies-literature
UnknownUsing mechanical homogenization to isolate microglia from mouse brain tissue to preserve transcriptomic integrity.
Not available
S-EPMC9485281 | biostudies-literature
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