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TranscriptomicsTranscription profiling of rat at pheochromocytoma cell line PC12 treated with trimethyltin at low and high doses
The rat pheochromocytoma cell line PC12 cells were cultured in complete DMEM till 80% confluence, then placed at 5000 cells per squared cm. Cells were then plated in 24-well plates for cell viability assay and in T75 flasks for RNA isolation. Medium was replaced with serum-free fresh medium for 12 ...
ORGANISM(S): Rattus norvegicus 
2009-04-29 | E-GEOD-5073 | biostudies-arrayexpress
TranscriptomicsExpression Analysis of Trimethyltin Chlorides (TMT) Impact on Embryonic Development
Investigation of whole genome expression pattern of 60 and 72 hours post fertilization Danio Rerio embryos exposed to TMT and vehicle control Embryos were exposed to 10uM TMT or control from 48hpf to 60 or 72 hpf. Three replicates were collected for each time point. 40 embryos were pooled to compris...
ORGANISM(S): Danio rerio 
2011-03-23 | E-GEOD-28131 | biostudies-arrayexpress
ProteomicsTMT-based Multiplexed (Chemo)proteomics on the Orbitrap Astral Mass Spectrometer
This dataset contains an evaluation of TMT-based multiplexed proteomics on the Orbitrap Astral mass spectrometer.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-12-17 | MSV000096674 | MassIVE
ProteomicsAnalysis of Reproducibility of Proteome Coverage and Quantitation Using Isobaric Mass Tags (iTRAQ and TMT)
This study aimed to compare the depth and reproducibility of total proteome and differentially expressed protein coverage in technical duplicates and triplicates using iTRAQ 4-plex, iTRAQ 8-plex and TMT 6-plex reagents.
ORGANISM(S): Phaeosphaeria Nodorum Sn15 (ncbitaxon:321614) 
2016-03-17 | MSV000079594 | MassIVE
ProteomicsOne-STAGE Tip Method for TMT based Proteomic Analysis of Minimal Amount of Cells
LC-MS-based profiling of proteomes with isobaric tag labeling from low-quantity biological and clinical samples, including needle-core biopsies and laser capture microdissection, has been challenging due to limited amount and sample loss during preparation. To address this problem, we developed OnM ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-04-18 | MSV000091734 | MassIVE
ProteomicsComparison of DIA and TMT based protein quantification in complex background
Label free quantification (LFQ) and isobaric labelling quantification (ILQ) are among the most popular protein quantification workflows in discovery proteomics. Here, we compared the TMT 10-plex workflow to label free single shot data-independent acquisition (DIA) method on a controlled sample set. ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2020-01-29 | MSV000084859 | MassIVE
TranscriptomicsMicroarray analysis of the effects of a γ-tocopherol-rich mixture of tocopherols on global gene expression in the prostates of TRAMP mice
We used whole mouse genome microarrays to the effects of γ-TmT on global gene expression in the prostate TRAMP and age-matched C57BL/6 mice were administered either 600 mg/kg of γ-TmT or a control vehicle via oral gavage. Twelve hours after dosing, prostate tissues were collected for RNA extracti...
ORGANISM(S): Mus musculus 
2014-09-16 | E-GEOD-61416 | biostudies-arrayexpress
ProteomicsMouse experiment TMT Proteomics
mouse experiment TMT Proteomics animal immunization
ORGANISM(S): Mus Musculus 
2025-12-05 | PXD071626 |
ProteomicsTMT labeling for the masses: A robust and cost-efficient, in-solution labeling strategy
Isobaric stable isotope labeling using, for example, tandem mass tags (TMTs) is increasingly being applied for large-scale proteomic studies. Experiments focusing on proteoform analysis in drug time course or perturbation studies or in large patient cohorts greatly benefit from the reproducible quan...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-01-14 | PXD012703 | Pride
Stable isotope labeling Tandem mass tags Nhs ester chemistry Labeling efficiency 
ProteomicsMultiplexed Proteome Profiling of Carbon Source Perturbations in Two Yeast Species with SL-SP3-TMT
Saccharomyces cerevisiae and Schizosaccharomyces pombe are the most commonly studied yeast model systems, yet comparisons of global proteome remodeling between these yeast species are scarce. Here, we profile the proteomes of S. cerevisiae and S. pombe cultured with either glucose or pyruvate as the...
ORGANISM(S): Homo sapiens (Human) 
2021-05-11 | PXD014546 | Pride
Sps-ms3 Cerevisiae Pombe Tmt Isobaric Pyruvate 
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