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MetabolomicsCell Membrane Remodeling Mediates Polymyxin B Resistance in Klebsiella pneumoniae: An Integrated Proteomics and Metabolomics Study
Polymyxin B (PB) is introduced into the clinic as the last-line therapy against carbapenem-resistant Klebsiella pneumoniae (CRKP). Unfortunately, increased resistance to PB in Klebsiella pneumoniae (K. pneumoniae) has threatened global health. Resistance of K. pneumoniae ...
2022-03-04 | MTBLS4132 | MetaboLights
ProteomicsTMT labeling for the masses: A robust and cost-efficient, in-solution labeling strategy
Isobaric stable isotope labeling using, for example, tandem mass tags (TMTs) is increasingly being applied for large-scale proteomic studies. Experiments focusing on proteoform analysis in drug time course or perturbation studies or in large patient cohorts greatly benefit from the reproducible quan...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-01-14 | PXD012703 | Pride
Stable isotope labeling Tandem mass tags Nhs ester chemistry Labeling efficiency 
UnknownA benchmarking protocol for intact protein-level Tandem Mass Tag (TMT) labeling for quantitative top-down proteomics.
Not available
S-EPMC9587358 | biostudies-literature
UnknownTMT Labeling for the Masses: A Robust and Cost-efficient, In-solution Labeling Approach.
Not available
S-EPMC6601210 | biostudies-literature
UnknownOptimization of protein-level tandem mass tag (TMT) labeling conditions in complex samples with top-down proteomics.
Not available
S-EPMC9371347 | biostudies-literature
UnknownAssessment of TMT Labeling Efficiency in Large-Scale Quantitative Proteomics: The Critical Effect of Sample pH.
Not available
S-EPMC8154127 | biostudies-literature
UnknownSPOT: spatial proteomics through on-site tissue-protein-labeling.
Not available
S-EPMC11515502 | biostudies-literature
ProteomicsNano-scale solid-phase isobaric labeling for multiplexed quantitative phosphoproteomics
We established a workflow for highly sensitive multiplexed quantitative phosphoproteomics using a nano-scale solid-phase tandem mass tag (TMT) labeling reactor. Phosphopeptides were firstly enriched by titanium oxide chromatography and then labeled with isobaric TMT reagents in a StageTip packed wit...
ORGANISM(S): Homo Sapiens (human) 
2021-07-23 | PXD025536 | JPOST Repository
ProteomicsOne-STAGE Tip Method for TMT based Proteomic Analysis of Minimal Amount of Cells
LC-MS-based profiling of proteomes with isobaric tag labeling from low-quantity biological and clinical samples, including needle-core biopsies and laser capture microdissection, has been challenging due to limited amount and sample loss during preparation. To address this problem, we developed OnM ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-04-18 | MSV000091734 | MassIVE
ProteomicsStreamlined TMT labeling workflow for proteomics and metaproteomics
This study established a streamlined TMT labeling workflow that is readily compatible with high throughput proteomics and metaproteomics sample preparation. The method was then evaluated using two different sample types, microbiome and intestinal Caco-2 cell line, with TMT11plex mix reagents.
ORGANISM(S): metagenome Homo sapiens (Human) 
2023-02-07 | PXD034936 | Pride
Proteomics Tmt Microbiome Metaproteomics 
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