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TranscriptomicsTranscriptome alterations associated with TMV-resistance in transgenic tobacco plants expressing the genomic RNA of the virus
Agilent 4x44k tobacco micro array of wild type tobacco (WT) and whole tobacco mosaic virus (TMV) containing transgenic tobacco plants. The transgenic plants before resistance break (BRB-6 weeks), after resistance break (ARB-8 weeks) and wild type tobacco plants infected with TMV (TMVi-9weeks) leaves...
ORGANISM(S): Nicotiana tabacum 
2014-11-03 | E-MEXP-3934 | biostudies-arrayexpress
TranscriptomicsTMV-derived Small RNAs Reveals Role for Arabidopsis thaliana RNA-dependent RNA Polymerases in Viral siRNA Biogenesis
Using a crucifer-infecting strain of Tobacco Mosaic Virus (TMV-Cg) and Arabidopsis thaliana as a model system, we analyzed the viral small RNA profile in wild-type plants as well as rdr mutants by applying small RNA deep sequencing technology. Over 100,000 TMV-Cg-specific small RNA reads, mostly of...
ORGANISM(S): Youcai mosaic virus 
2009-03-24 | E-GEOD-12918 | biostudies-arrayexpress
TranscriptomicsArabidopsis protoplasts : TMV*rep vs. TMV*CP.MP transfected
Transcriptional profiling of Arabidopsis in response to infection with TMV in the initial infection stage (0.5, 4 and 6 hours post inoculation). Analyze the relative transcriptome change of TMV*CP.MP- and TMV*rep-inoculated samples. TMV*rep vs. TMV*CP.MP transfected cells. 2 biological replicates co...
ORGANISM(S): Arabidopsis thaliana 
2013-04-15 | E-GEOD-45283 | biostudies-arrayexpress
TranscriptomicsExpression data from transgenic Nicotiana tabacum expressing a variant of coat protein and movement protein from Tobacco mosaic virus
Transgenic expression of viral proteins in natural host plants is a useful simplified system with the potential to understand the individual effect of each viral component. Transgenic expression of movement (MP) and a variant from coat protein (CPT42W) in tobacco, a TMV natural host, produces sever...
ORGANISM(S): Nicotiana tabacum 
2012-12-01 | E-GEOD-37905 | biostudies-arrayexpress
TranscriptomicsProfiling of genes related to cross protection and competition for NbTOM1 by HLSV and TMV
To investigate the mechanism under the cross protection between HLSV and TMV, microarray analysis was conducted to examine the transcriptional levels of global host genes during cross protection, using Tobacco Gene Expression Microarray, 4x44k slides. The transcriptional level of some host genes cor...
ORGANISM(S): Nicotiana benthamiana 
2014-05-12 | E-GEOD-47180 | biostudies-arrayexpress
ProteomicsProteomic analysis of early stage infection in the tobacco-Tobacco mosaic virus (TMV)
The early events of virus infection is one of the more poorly understood areas of plant virology and studies on the effect of virus on the host proteome at very early stages of infection are lacking. In the present study, we analysed the proteome on the early stage changes at 15 minutes post inocula...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2021-05-12 | PXD022517 | Pride
Tmv 
ProteomicsDifferential regulation of tobacco host factors upon TMV infection
Tobacco mosaic virus (TMV) became one of the model virus for fundamental biological research and understanding of the host-pathogen interaction. It utilizes host machinery smartly to complete the life cycle, though many parts of life cycle remained a mystery, such as host factors involved in virus r...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2018-11-07 | PXD008837 | Pride
Tobacco Tmv Itraq 
ProteomicsCrosstalk of Protein Expression and Lysine Acetylation in Response to TMV Infection in Nicotiana benthamiana
Kac, a reversible PTM, plays essential roles in various biological processes, including those involving metabolic pathways, pathogen resistance and transcription, in both prokaryotes and eukaryotes. TMV, the major factor that causes poor quality of Solanaceae crops worldwide, directly alters many me...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2022-10-15 | PXD035737 | Pride
Lysine acetylation Tmv Nicotiana benthamiana Proteome 
ProteomicsiTRAQ based analysis of leaf proteome identifies important proteins in secondary metabolite biosynthesis and defence pathways crucial to cross-protection against TMV
We used milder strain of TMV mutant (TMV-43A) to cross protect Nicotiana benthamiana plants from TMV. We used iTRAQ (2 sets) quantitative proteomics approach to investigate molecular responses and identified important host proteins from secondary metabolite biosynthesis and defence pathways
ORGANISM(S): Nicotiana benthamiana 
2018-11-21 | PXD011633 | Pride
Cross-protection Secondary metabolite biosynthesis Tmv Itraq Nicotiana benthamiana 
ProteomicsProteomic analysis of TMV and TMV 24A+UPD infected N. benthamiana plants by iTRAQ reveal differences in stress signalling, cell death and response to intracellular iron
TMV was mutated by introducing different lengths of internal poly (A) tract in front of the upstream pseudo knotted domain .Twenty four nucleotides were inserted into the TMV genome, this mutant is designated TMV 24A +UPD TMV 24+UPD shows more severe leaf necrosis and stem collapse, leading to plant...
ORGANISM(S): Nicotiana benthamiana 
2019-10-17 | PXD011825 | Pride
Tmv Tmv 24a+upd 
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