Lipidomic profiling was performed to compare control, SCD1-knockout, and SCD2-knockout C2C12 cells under standard culture conditions. Cell pellets were collected from routinely cultured cells, rapidly frozen, and stored at −80°C prior to lipid extraction and LC–MS/MS analysis. The study aimed to ...

Ubiquitin-like protein ISG15 (interferon-stimulated gene 15) has been implicated in the regulation of central carbon metabolism, but conflicting findings across experimental systems have limited mechanistic insight. Here, we applied a multi-omics approach in cells ectopically expressing the ISGyl...

The gut microbiota is increasingly recognized for playing a critical role in human health and disease, especially in conferring resistance to both virulent pathogens such as Salmonella, which infects 1.2 million people in the United States every year [1], and opportunistic pathogens like Candida,...

To elucidate the metabolic response of Brevibacterium epidermidis TRM83610 to NaCl stress and facilitate its industrial application, this study employed metabolomics techniques to analyze changes in intracellular metabolites, particularly compatible solutes, under NaCl concentrations of 0, 5%, 10...