Sort   by:  
 Page size 
ProteomicsIdentification of sulfhydryl-containing proteins and further evaluation of the selenium- tagged redox homeostasis-regulating proteins:Identification of cysteine sites using tandem orthogonal proteolysis strategy (TOP-ABPP).
We sought to identify exact modified cysteine sites by Se-probes using TOP-ABPP strategy and the acid cleavable azide-biotin tag was used to enrich and release the modified peptides for LC-MS/MS analys
ORGANISM(S): Homo sapiens (Human) 
2023-12-07 | PXD046852 | Pride
Top-abpp npspyne npssyne dpdsyne 
ProteomicsA Simplified and Ultrafast Pipeline for Site-specific Quantitative Chemical Proteomics
Activity-based proteome profiling (ABPP) is a powerful chemoproteomic technology for global profiling of protein reactivity and modifications. The tandem orthogonal proteolysis-ABPP (TOP-ABPP) strategy utilizes a clickable enrichment tag with cleavable linkers to enable direct identification of prob...
ORGANISM(S): Homo Sapiens 
2023-03-15 | PXD040880 |
UnknownIdentification of sulfhydryl-containing proteins and further evaluation of the selenium-tagged redox homeostasis-regulating proteins.
Not available
S-EPMC10755098 | biostudies-literature
UnknownChemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe.
Not available
S-EPMC5390668 | biostudies-literature
ProteomicsHaloacetic Acids and Haloacetamides Attack Distinct Cellular Proteome Thiols
Disinfection by-products (DBPs) exposure has been linked to multiple adverse health outcomes. However, the molecular initiating events by which DBPs induce their toxicities remains unclear. Herein we combined reporter cell lines and activity-based protein profiling (ABPP) chemical proteomics to iden...
ORGANISM(S): Homo sapiens (Human) 
2020-11-11 | PXD018825 | Pride
Disinfection by-products  proteomics  protein adducts  gapdh  nrf2-keap1 
TranscriptomicsComparative transcriptome profiling of Amyloid Precursor Protein APP family members in the adult cortex
The β-amyloid precursor protein APP and the related APLPs, undergo complex proteolytic processing giving rise to several fragments. Whereas it is well established that Aβ accumulation is a central trigger for Alzheimer disease (AD), the physiological role of APP family members and their diverse pr...
ORGANISM(S): Mus musculus 
2011-03-18 | E-GEOD-25926 | biostudies-arrayexpress
ProteomicsDegrasyn exhibits antibiotic activity against multi-resistant Staphylococcus aureus by modifying several essential cysteines
In this dataset, we investigate the targets of degrasyn in the methicillin-sensitive S. aureus strain NCTC 8325. This includes data on enrichment studies and competition studies with conventional ABPP using a degrasyn-derived probe, competition studies with residue-specific proteomics using the isoD...
ORGANISM(S): Staphylococcus aureus 
2020-04-14 | PXD016413 | Pride
Chemoproteomics Antibioitcs S. aureus Isodtb-abpp 
ProteomicsA quantitative map of beta-lactone induced virulence regulation
Beta-lactones have recently been introduced as the first selective ClpP inhibitors that attenuate virulence of both sensitive Staphylococcus aureus and multiresistant strains (MRSA). Although previous knockout studies showed that ClpP is essential for S. aureus alpha-toxin production a link between ...
ORGANISM(S): Staphylococcus aureus 
2017-02-08 | PXD004680 | Pride
Proteomics Chemical knockout S. aureus clpp Antivirulence Activity-based protein profiling 
ProteomicsDeubiquitinase Vulnerabilities Identified through Activity Based Protein Profiling in Non-Small Cell Lung Cancer
To aid in the prioritization of deubiquitinases (DUBs) as anticancer targets, we developed an approach combining activity-based protein profiling (ABPP) with mass spectrometry in both non-small cell lung cancer (NSCLC) tumor tissues and cell lines along with analysis of available RNA interference an...
ORGANISM(S): Homo sapiens (Human) 
2022-07-05 | PXD028802 | Pride
Human Activity-based protein profiling Deubiquitinase Lung cancer 
ProteomicsReimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries
Current methods for measuring amino-acid side-chain reactivity lack the throughput needed to screen large chemical libraries for interactions across the proteome. Here we redesigned the workflow for activity-based protein profiling of reactive cysteine residues by using a smaller desthiobiotin-based...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-01-26 | PXD022511 | Pride
Reactive cysteines Chemical proteomics 
Sort   by:  
 Page size