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MetabolomicsDifferent regions of synaptic vesicle membrane regulate VAMP2 conformation for the SNARE assembly
Vesicle associated membrane protein 2 (VAMP2/synaptobrevin2), a core SNARE protein residing on synaptic vesicles (SVs), forms helix bundles with syntaxin-1 and SNAP25 for the SNARE assembly. Prior to the SNARE assembly, the structure of VAMP2 is unclear. Here, by using in-cell NMR spectroscopy, we d...
2021-10-06 | MTBLS1503 | MetaboLights
MetabolomicsLION-web validation lipidomics experiments (FA-incorporation, membrane fluidty)
A major challenge for lipidomic analyses is the handling of the large amounts of data and the translation of results to interpret the involvement of lipids in biological systems. We built a new lipid ontology (LION) that associates over 50,000 lipid species to biophysical, chemical and cell biologic...
2019-04-24 | MTBLS757 | MetaboLights
MetabolomicsControlling mitochondrial membrane architecture via MIC60 determines viral replication to promote anti-viral immunity
Virus-infected cells often exhibit dramatic cellular changes accompanied by altered mitochondrial function. The contribution of factors shaping the inner mitochondrial membrane (IMM) and cristae architecture on viral replication is insufficiently understood. Single cell transcriptomics applying vesi...
2025-06-24 | MTBLS12437 | MetaboLights
TranscriptomicsMembrane-polysome associated fractionation of HES2 embryonic stem cells
The membrane fraction and the cytosolic fraction of HES2 cells were collected and subjected to microarray. The experiment was performed in triplicate Keywords: membrane-polysome fractionation of RNA Three consecutive passages of HES2 cells were grown and subject to membrane-polysome fractionation....
ORGANISM(S): Homo sapiens 
2010-04-07 | E-GEOD-14037 | biostudies-arrayexpress
TranscriptomicsGenome-Scale Identification of Membrane-Associated Human mRNAs
The subcellular localization of proteins is critical to their biological roles. Moreover, whether a protein is membrane-bound, secreted, or intracellular affects the usefulness of, and the strategies for, using a protein as a diagnostic marker or a target for therapy. We employed a rapid and efficie...
ORGANISM(S): Homo sapiens 
2006-02-24 | E-GEOD-4175 | biostudies-arrayexpress
ProteomicsThe Whole cell lysate and Crude membrane of HEK293T cells
We perform the experiment in order to compare identified membrane proteins from whole cell lysate and membrane fraction in HEK293T cell. The isolation of membrane fractions is critical to obtain a more comprehensive membrane proteome, especially with proteins containing transmembrane domains.
ORGANISM(S): Homo Sapiens (human) 
2022-04-28 | PXD027472 | JPOST Repository
ProteomicsLFQ comparison of total cell lysate, lysosomal and plasma membrane fraction of WT and NPC1 KO cells
Superparamagnetic iron oxide nanoparticles (SPIONs) have so far mainly been used as cellular carriers for genes and therapeutic products, while their use in subcellular organelle isolation remains largely underexploited. We engineered surface functionalized SPIONs (Ø 10 nm) that target very distinc...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080792 | MassIVE
TranscriptomicsRNA-Seq of human hematopoietic stem and progenitor cells to study membrane repair following electroporation (EP) or filtroporation (FP) disruption based strategies for intracellular delivery
This experiment investigates how different intracellular delivery methods, specifically filtroporation (FP) and electroporation (EP) affect gene editing efficiency and cellular responses in human hematopoietic stem and progenitor cells. By comparing these methods, results show how mechanical versus ...
ORGANISM(S): Homo sapiens 
2025-10-30 | E-MTAB-15741 | biostudies-arrayexpress
ProteomicsEnhancing Membrane Protein Identification Using a Simplified, Centrifugation and Detergent-based Membrane Extraction Approach
Membrane proteins may act as transporters, receptors, enzymes and adhesion-anchors, accounting for nearly 70% of pharmaceutical drug targets. Difficulties in efficient enrichment, extraction and solubilization still exist because of their relatively low abundance and poor solubility. A simplified me...
ORGANISM(S): Mus musculus (Mouse) 
2019-09-27 | PXD007731 | Pride
Sequence coverage Identification Mass spectrometry Enrichment Membrane proteins 
ProteomicsMembrane modulation of super-secreting “midiBacillus” expressing the major Staphylococcus aureus antigen – a mass-spectrometry based absolute quantification approach.
Bacillus subtilis is a Gram-positive bacterium considered as a “cell-factory” for industrial enzymes and biopharmaceuticals. Given the commercial advantage of this organism, researchers have been working towards the improvement of this organism as a producer of secreted proteins. Nonetheless, there ...
ORGANISM(S): Bacillus subtilis subsp. subtilis 
2020-03-20 | PXD015496 | Pride
Srm Membrane proteins Heterologous protein production Proteomics Absolute quantification 
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