To identify proteins regulated by TRMT6/TRMT61A complex, we depleted TRMT6/TRMT61A in human liver CSCs, and compared the differential proteins with control CSCs by LC-MS/MS.
In order to identify the molecular mechanism leading to the increase of TRMT6/61A complex in aged HSCs, we purified proteins interacting with TRMT6 and TRMT61A via affinity purification. HEK293T cells stably expressing SFB-tagged TRMT6 or SFB-TRMT61A were lysed in NETN buffer (20 mM Tris-HCl [pH 8.0...
This study investigates the transcriptomic changes in DLD1 colon cancer cells upon overexpression of TRMT6 and TRMT61A genes, with a focus on identifying downstream genes associated with tumor cell senescence. We performed RNA-seq, tRNA m1A-MeRIP-seq, and ribo-seq on DLD1 cells overexpressing TRMT6 ...
This study investigates the transcriptomic changes in DLD1 colon cancer cells upon overexpression of TRMT6 and TRMT61A genes, with a focus on identifying downstream genes associated with tumor cell senescence. We performed RNA-seq, tRNA m1A-MeRIP-seq, and ribo-seq on DLD1 cells overexpressing TRMT6 ...
TRMT6/61A complex methylates N1-adenine in tRNAs and mRNAs. We analyzed disturbances of proteome homeostasis in human cells when N1-methyladenine (m1A) generation is disturbed due to the deficiency of TRMT61A.
Increased protein translation plays a critical role in cancer development and treatment1,2. However, the molecular mechanism that is involved in this process remains poorly understood. N1-methyladenosine (m1A) methylation in RNA accounts for regulating mRNA translation in a post-transcriptional mann...
