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ProteomicsSequential Phosphoproteomic Enrichment by Complementary Metal-directed Immobilized Metal Ion Affinity Chromatography: Case Study on Kinase Substrate Mapping in Human Lung Cancer Tissue
Abstract: Despite recent advances in instrumentation and analytical strategies for identification and quantitation of protein phosphorylation, methodologies to enrich the heterogeneous types of phosphopeptides are critical towards comprehensive mapping of the under-explored phosphoproteome. Taking a...
ORGANISM(S): Homo sapiens (Human) 
2014-01-29 | PXD000314 | Pride
Human lung cancer tissue Sequential imac Phosphoproteomics 
ProteomicsHs_Lung_Phospho_SequentialIMAC_tissue
Sequential Phosphoproteomic Enrichment by Complementary MD-IMAC, Cancer tissues
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005323 | PeptideAtlas
ProteomicsHs_Lung_Phospho_SequentialIMAC_cell
Sequential Phosphoproteomic Enrichment by Complementary MD-IMAC, Raji cell line
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005320 | PeptideAtlas
UnknownMUSE and PROPELLER DWI for ADC in parasagittal dura: insights from high-resolution and reduced-distortion DWI.
Not available
S-EPMC11876650 | biostudies-literature
ProteomicsA streamlined tandem tip-based workflow for sensitive nanoscale phosphoproteomics
Effective nanoscale phosphoproteome analysis remains a daunting task, primarily due to significant sample loss associated with non-specific surface adsorption during phosphopeptide enrichment and other sample processing steps. To address this issue, we have developed a tandem tip-based C18-IMAC-C18 ...
ORGANISM(S): Homo Sapiens (human) 
2023-01-27 | PXD032019 | JPOST Repository
ProteomicsLarge-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics
We developed a motif-based isotopic quantitative method for determination of phosphorylation stoichiometry based on kinase selection.
ORGANISM(S): Homo sapiens (Human) 
2015-03-30 | PXD000728 | Pride
Motif Imac Drug resistance Phosphorylation stoichiometry Lung cancer 
ProteomicsPhosphopattern Predicts CK2 Substrate, HMGA1, as a Drug Resistant Target for Non-Small Cell Lung Cancer
Lung adenocarcinoma cells harboring epidermal growth factor receptor (EGFR) mutations are sensitive to EGFR tyrosine kinase inhibitors (TKIs), including gefitinib. Acquired resistance to EGFR-TKIs develops after prolonged treatments. Known mechanisms for EGFR-TKI resistance, including KRAS mutation,...
ORGANISM(S): Homo sapiens (Human) 
2018-05-08 | PXD000861 | Pride
Imac Phosphoproteome Hmga1 Drug resistance Ck2 Lung cancer 
ProteomicsUntargeted data independent acquisition proteomics analysis using Orbitrap mass spectrometers and DIA-Umpire
Untargeted data independent acquisition proteomics analysis using Orbitrap mass spectrometers and DIA-Umpire
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-04-01 | MSV000080875 | MassIVE
ProteomicsSequential Phosphoproteomic Enrichment through Complementary Metal-Directed Immobilized Metal Ion Affinity Chromatography
We developed a tip-based sequential IMAC based on different kinds of metal ion for increasing the coverage of phosphoproteome. This approach not only can be used for singly and multiply phosphopeptides separation but also for acidic, basophilic and Pro-directed phosphopeptides separation.
ORGANISM(S): Homo Sapiens (human) 
2016-09-07 | PXD004903 | JPOST Repository
ProteomicsNano-scale solid-phase isobaric labeling for multiplexed quantitative phosphoproteomics
We established a workflow for highly sensitive multiplexed quantitative phosphoproteomics using a nano-scale solid-phase tandem mass tag (TMT) labeling reactor. Phosphopeptides were firstly enriched by titanium oxide chromatography and then labeled with isobaric TMT reagents in a StageTip packed wit...
ORGANISM(S): Homo Sapiens (human) 
2021-07-23 | PXD025536 | JPOST Repository
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