Sort   by:  
 Page size 
TranscriptomicsTU-tagging: cell type specific RNA isolation from intact complex tissues
We show that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil (4TU) delivery can be used to label and purify cell type specific RNA from intact complex tissues. This method is useful for isolating RNA from cell types that are difficult to i...
ORGANISM(S): Drosophila melanogaster 
2010-06-19 | E-GEOD-15445 | biostudies-arrayexpress
ProteomicsThe Shewanella oneidensis Fic enzyme SoFic targets theswitch-I region of EF-Tu for AMPylation
Fic enzymes mediate diverse post-translational modifications, including adeno-sine monophosphate (AMP) transfer and removal, referred to as AMPylationand deAMPylation, respectively. We identified the prokaryotic translationelongation factor Tu (EF-Tu) as an AMPylation target of the Fic enzymeSoFic. ...
ORGANISM(S): Escherichia coli 
2026-09-18 | PXD071331 | Pride
Structural regulation Ampylation Ef-tu Fic enzymes Deampylation 
ProteomicsIn vitro synthesis of 32 translation-factor proteins from a single template reveals impaired ribosomal processivity
The Protein synthesis Using Recombinant Elements (PURE) system enables transcription and translation of a DNA template from purified components. Therefore, the PURE system-catalyzed generation of RNAs and proteins constituting the PURE system itself represents a major challenge toward a self-replica...
ORGANISM(S): Escherichia Coli 
2021-09-06 | PXD020877 | panorama
MetabolomicsDiscovery of urinary biomarkers to discriminate between exogenous and semi-endogenous thiouracil in cattle (Calf samples)
In the European Union, the use of thyreostats for animal fattening purposes has been banned because of their carcinogenic and teratogenic effects, whereby any residues in derived food products may hold a risk for human health. In this regard, potential abuse is monitored by targeted analysis of thyr...
2018-02-22 | MTBLS554 | MetaboLights
MetabolomicsDiscovery of urinary biomarkers to discriminate between exogenous and semi-endogenous thiouracil in cattle (Cow samples)
In the European Union, the use of thyreostats for animal fattening purposes has been banned because of their carcinogenic and teratogenic effects, whereby any residues in derived food products may hold a risk for human health. In this regard, potential abuse is monitored by targeted analysis of thyr...
2018-02-22 | MTBLS561 | MetaboLights
TranscriptomicsTranslational regulation of Anopheles gambiae mRNAs in the midgut during Plasmodium falciparum infection
Malaria is caused by Plasmodium parasites, which are transmitted via the bites of infected Anopheline mosquitoes. Midgut invasion is a major bottleneck for Plasmodium development inside the mosquito vectors as a rapidly responding immune system recognizes ookinetes and recruits killing factors from ...
ORGANISM(S): Anopheles gambiae 
2012-09-21 | E-GEOD-38707 | biostudies-arrayexpress
Transcriptomicsc-myc-3'RR/p53+/- mice lymphomas
c-myc-3'RR mice prone to develop Burkitt lymphoma (BL) were crossed with p53+/- mice in order to obtain c-myc-3'RR/p53+/- mice. These mice develop a wider spectrum of lymphoma including BL, mantle cell lymphoma (MCL) and plasma cell lymphoma (PCL). Transcriptoma analysis of these lymphomas is invest...
ORGANISM(S): Mus musculus 
2012-01-05 | E-GEOD-31814 | biostudies-arrayexpress
UnknownEF-Tu From Non-typeable Haemophilus influenzae Is an Immunogenic Surface-Exposed Protein Targeted by Bactericidal Antibodies.
Not available
S-EPMC6305414 | biostudies-literature
ProteomicsQuantitative photo-crosslinking mass spectrometry reveals protein structure response to environmental changes
Conformational changes of protein structures depend on their chemical and physical environment. Studying conformational changes depending on environmental parameters is notoriously difficult as many methods of structural biology are affected by parameters like temperature or pH. To make such conform...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2019-07-01 | PXD012939 | Pride
Data-independent acquisition Structural dynamics Human serum albumin Dia-qclms Quantitation Xi Cytochrome c Spectronaut Label-free Mass spectrometry Photo-crosslinking Cross-linking Sda 
UnknownProteomic Analysis Revealed Metabolic Inhibition and Elongation Factor Tu Deamidation by p-Coumaric Acid in Cronobacter sakazakii.
Not available
S-EPMC9125189 | biostudies-literature
Sort   by:  
 Page size