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ProteomicsUsing L3MBTL3 knockout and rescued stable human breast cancer cell line (MDA-MB-231) to detect the novel interacting proteins of L3MBTL3 by immunoaffinity purification and mass spectrometry. Identification of L3MBTL3 interaction proteins in the breast cancer cell.
We used L3MBTL3 knockout MDA-MB-231 cell (L3-KO5+V) and L3MBTL3 rescue MDA-MB-231 cell (L3-KO5+L3). The cells were collected by centrifugation and lysed in lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.1% NP40, 8% glycerol, protease inhibitor cocktail (#P1010, Beyotime, China) on i...
ORGANISM(S): Homo sapiens (Human) 
2024-10-25 | PXD040150 | Pride
Human Breast cancer cell L3mbtl3 
GenomicsWhole-genome study reveals distinct mechanisms used by p53 to regulate activated and repressed genes in embryonic stem cells
This SuperSeries is composed of the following subset Series: GSE26360: Genome-wide analysis revealed a crosstalk between p53 and the pluripotent gene networks in mouse embryonic stem cells (expression) GSE26361: Genome-wide analysis revealed a crosstalk between p53 and the pluripotent gene networks ...
ORGANISM(S): Mus musculus 
2012-03-05 | E-GEOD-26362 | biostudies-arrayexpress
TranscriptomicsGenome-wide analysis revealed a crosstalk between p53 and the pluripotent gene networks in mouse embryonic stem cells (expression)
The tumor suppressor p53 regulates the differentiation of embryonic stem (ES) cells upon DNA damage. However, our understanding of this critical tumor suppressive role of p53 in ES cells is limited, mainly because of the lack of molecular mechanism. Here, we report a widespread cross-regulation of p...
ORGANISM(S): Mus musculus 
2012-03-05 | E-GEOD-26360 | biostudies-arrayexpress
GenomicsGenome-wide analysis revealed a crosstalk between p53 and the pluripotent gene networks in mouse embryonic stem cells (ChIP-Seq)
Study of the function of p53 in regulating gene expression in mouse embryonic stem cells. It is critical for embryonic stem cells to maintain their genomic stability. The guardian of the genome, p53, plays important roles in maintaining the genomic integrity of ES cells through regulating the differ...
ORGANISM(S): Mus musculus 
2012-03-05 | E-GEOD-26361 | biostudies-arrayexpress
TranscriptomicsRegulatory RNA Apela Is a Component of a Negative Feedback Loop Regulating p53-Mediated Apoptosis in Embryonic Stem Cells (II)
The goal is to identify regulatory RNAs that regulate the p53 signaling pathway in embryonic stem (ES) cells. p53+/+ mES cells and p53-/- mES cells were untreated or treated with 0.5 uM Adriamycin for 8 hours. Total RNA was extracted and subject to RNAseq.
ORGANISM(S): Mus musculus 
2015-06-05 | E-GEOD-60843 | biostudies-arrayexpress
TranscriptomicsRegulatory RNA Apela Is a Component of a Negative Feedback Loop Regulating p53-Mediated Apoptosis in Embryonic Stem Cells (I)
p53-repressed transcripts have recently been shown to play important roles in various biological processes, such as stem cell differentiation and cancer. We identified a transcript named Apela that is repressed by p53 and highly expressed in mouse ES cells. To see which transcripts are affected by A...
ORGANISM(S): Mus musculus 
2015-06-05 | E-GEOD-54355 | biostudies-arrayexpress
TranscriptomicsRegulatory RNA Apela Is a Component of a Negative Feedback Loop Regulating p53-Mediated Apoptosis in Embryonic Stem Cells (III)
The goal is to identify RNAs that bind to Hnrnpl (hnRNP L) using RNA immunoprecipitation (RIP) assay. p53+/+ mES cells and p53-/- mES cells were untreated or treated with 0.5 uM Adriamycin for 8 hours. RNA immunoprecipitation was performed using Hnrnpl antibody (Bethyl, Cat#: A311-423A). Total RNA w...
ORGANISM(S): Mus musculus 
2015-06-05 | E-GEOD-62378 | biostudies-arrayexpress
UnknownThe Potential Mechanism of Wuwei Qingzhuo San against Hyperlipidemia Based on TCM Network Pharmacology and Validation Experiments in Hyperlipidemia Hamster.
Not available
S-EPMC7212318 | biostudies-literature
ProteomicsDNA damage-induced alternative splicing of p53
Samples were reduced in loading buffer and separated by SDS-PAGE for 3-4 min followed by in-gel digestion with trypsin. Digests were analyzed by LC-MS/MS using a Waters nanoACQUITY interfaced to a Thermo Fusion Lumos MS. The LC separation 90 min gradient of 5-30% MeCN in a trapping configurations w...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-12-30 | MSV000086651 | MassIVE
UnknownThree-Dimensional Multimodality Image Reconstruction as Teaching Tool for Case-based learning among medical postgraduates: a focus on primary pelvic bone Tumour Education.
Not available
S-EPMC10717389 | biostudies-literature
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