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In this study, we identified ubiquitinated peptides that changed abundance in the mutant of an E3 ubiquitin ligase gene by coupling a K-(GG) peptide immunoprecipitation with quantitative proteomic analysis. Two independent replicates identified 265 and 236 ubiquitinated peptides, respectively. Seven...
ORGANISM(S): Solanum lycopersicum 
2020-11-24 | PXD018707 | Pride
This study investigates the impact of mild hypothermic conditions (31°C) on the whole cell proteome and ubiquitinated proteome of Chinese hamster ovary (CHO) cells, with a specific focus on endoplasmic reticulum (ER) proteins and ER stress. Using high-resolution mass spectrometry, we conducted a com...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2025-12-22 | PXD063085 | Pride
Trypsin specifically cleaves the C-terminus of lysine and arginine residues and is assumed to fails to cleave ubiquitin-modified sites. Based on this perception, the irregularly-cleaved ubiquitinated peptides were often regarded as false positives and discarded in proteomic analysis. Interestingly, ...
ORGANISM(S): Homo Sapiens 
2023-01-10 | PXD039353 |
In this study, we describe an antibody-based approach to enrich ubiquitinated peptides from vegetative tissues for detection via peptide mass spectrometry. This enrichment method can be coupled with isobaric labeling to enable quantification from up to 18-multiplexed samples. This approach identifie...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-07-31 | MSV000095496 | MassIVE
Ubiquitinated peptides (KGG peptides) purified from the yeast strains with or without mutation on the N-myristoylation site of the proteasome Rpt2 subunit were analyzed by an LTQ-Orbitrap Velos. The MS data was analyzed by the Progenesis LC-MS software.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-08-25 | PXD002274 | Pride
To identify ubiquitinated modified proteins of lung adenocarcinoma, we collected five pairs of lung adenocarcinoma and normal lung tissues from the clinic for analysis
ORGANISM(S): Homo sapiens (Human) 
2022-01-12 | PXD027001 | Pride
We performed diGLY enrichment followed by label-free proteomics in C. elegans to define and quantify changes in the ubiquitinated (Ub)-proteome during the aging process. More specifically, we compared wild-type worms at the first day of adulthood with young (day 5), mid-age (day 10) and aged adults ...
ORGANISM(S): Caenorhabditis elegans 
2021-05-27 | PXD024338 | Pride
Arsenic contamination in food and ground water constitutes a public health concern to more than 100 million people worldwide. Individuals chronically exposed to arsenic through drinking and ingestion exhibit a higher risk in developing cancers and cardiovascular diseases. Nevertheless, the underlyin...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD035401 | Pride
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