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ProteomicsMudPIT analyses of the proteins co-purified with wild type-Hzg-3XHA/Flag and phosphatase dead-Hzg-3XHA/Flag affinity purifications from Drosophila melanogaster S2 cells
Protein Complexes Purification- Stable S2 cell expressing Hzg albeit low level, lines expressing C-terminally 3XHA-Flag-tagged wild-type and phosphatase dead (PD) Hzg were generated. A parental cell line was used as a negative control. Nuclear and cytoplasmic extracts from the Hzg-WT, Hzg-mutant and...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2019-01-08 | MSV000083305 | MassIVE
ProteomicsHalo-SPIN1_stable-cell-line_Halo-AP-MudPIT
Halo-SPIN1 stable cell line clone #9 & #11 in 293FRT cells Blank 293FRT control Halo purification Lys-C;Trypsin LTQ MudPIT
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-11 | MSV000084679 | MassIVE
ProteomicsHalo-C11orf84_transient-transfection_Halo-AP-MudPIT
Transient transfection 293FRT cells Halo-C11orf84 and Halo-SNAP control Halo Purification Lys-C;Trypsine LTQ-MudPIT
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-11 | MSV000084678 | MassIVE
ProteomicsHalo-SPIN1/SNAP-SPINDOC SNAP-Halo-SCAP E1/UB2/E2-MudPIT
Halo-SPIN1 SNAP-SPINDOC Seriel capture affinity purified E1;UB2;E2 MudPIT
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-17 | MSV000084713 | MassIVE
ProteomicsHalo-SPIN1/SNAP-SPINDOC SNAP-Halo-SCAP E2-DSSO-XL
Halo-SPIN1 SNAP-SPINDOC Seriel Capture Affinity Purified E2; on-beads DSSO crosslinking ms1/ms2/ms3 PD export (combined)
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-18 | MSV000084719 | MassIVE
ProteomicsWDR76_SPIN1_Halo-AP_Enriched_Histones_AscendDIA
Histone PTM bottom-up profiling of Halo-WDR76 or Halo-SPIN1 Enriched or total histones (w Propionylation; no spike-in peptide) Supplementry data for WDR76-SPIN1 SCAP Analysis
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-04-15 | MSV000094548 | MassIVE
ProteomicsMudPIT LiPMS analyses of whole cell protein extracts from Saccharomyces cerevisiae grown at 21C and 35C
Sample Preparation. Three biological replicates of wild type yeast cells (BY4741) were cultured with YEPD either at 21C or 35C overnight (ca. 18hrs) to OD600 around 2. Pre-warmed/chilled fresh YEPD was next used to dilute the cultures, which were grown for an additional 6hrs to OD600 around 1. 120m...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2021-02-01 | MSV000086790 | MassIVE
ProteomicsHalo-WDR76_Purification_WDR76KObackground_MudPIT_wPTMs
Halo purification samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits Halo-WDR76. Purification was performed using whole cell extract from a stable cell line of 293FRT cells in WDR76 KO background. Control purification was performed on bl...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-11 | MSV000086849 | MassIVE
ProteomicsHalo-Control/WDR76/SPIN1/CBX5_purification_MudPIT_wPTMs
Halo purification samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits are Halo-WDR76, Halo-SPIN1, Halo-CBX5. The bait for Control Purification is Halo tag only. Purification for each bait was performed using whole cell extract from a stab...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-11 | MSV000086846 | MassIVE
ProteomicsHalo-WDR76_Purification_inWDR76KO_DSSO-XL-MS
Halo purified WDR76 from a stable cell line of 293FRT in WDR76 KO background. DSSO crosslinking performed in eluted samples. Data collected using ms1/ms2/ms3 method. Data searched using Proteomics Discoverer 2.4 with XlinkX.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-09 | MSV000086828 | MassIVE
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