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XL-MS mass spectrometry analysis Cross-linked peptides were resolved for mass spectrometry analysis using a Dionex UltiMate 3000 RSLnano liquid chromatography system. Peptides were initially loaded from the autosampler onto an Acclaim PepMap 100 C18 LC Trap Cartridge (0.3 mm inside diameter, 5 mm le...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-04-10 | MSV000087174 | MassIVE
Halo purification samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits Halo-WDR76. Purification was performed using whole cell extract from a stable cell line of 293FRT cells in WDR76 KO background. Control purification was performed on bl...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-11 | MSV000086849 | MassIVE
Halo purification samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits are Halo-WDR76, Halo-SPIN1, Halo-CBX5. The bait for Control Purification is Halo tag only. Purification for each bait was performed using whole cell extract from a stab...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-11 | MSV000086846 | MassIVE
Halo-SPIN1 stable cell line clone #9 & #11 in 293FRT cells Blank 293FRT control Halo purification Lys-C;Trypsin LTQ MudPIT
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-11 | MSV000084679 | MassIVE
Transient transfection 293FRT cells Halo-C11orf84 and Halo-SNAP control Halo Purification Lys-C;Trypsine LTQ-MudPIT
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-11 | MSV000084678 | MassIVE
Halo-SPIN1 SNAP-SPINDOC Seriel capture affinity purified E1;UB2;E2 MudPIT
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-17 | MSV000084713 | MassIVE
Halo-SPIN1 SNAP-SPINDOC Seriel Capture Affinity Purified E2; on-beads DSSO crosslinking ms1/ms2/ms3 PD export (combined)
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-18 | MSV000084719 | MassIVE
Sample Preparation. Three biological replicates of wild type yeast cells (BY4741) were cultured with YEPD either at 21C or 35C overnight (ca. 18hrs) to OD600 around 2. Pre-warmed/chilled fresh YEPD was next used to dilute the cultures, which were grown for an additional 6hrs to OD600 around 1. 120m...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2021-02-01 | MSV000086790 | MassIVE
Serial Capture Affinity Purification (SCAP) samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits are SNAP-SPIN1 and Halo-WDR76. SNAP purification was performed first, then Halo purification was performed. SCAP was performed using whole cel...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-03 | MSV000086802 | MassIVE
Histone PTM bottom-up profiling of Halo-WDR76 or Halo-SPIN1 Enriched or total histones (w Propionylation; no spike-in peptide) Supplementry data for WDR76-SPIN1 SCAP Analysis
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-04-15 | MSV000094548 | MassIVE
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