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Proteomicsfull length protein sequencing method based on less specific and unspecific hydrolysis strategies 2
We describe a new strategy for LC-MS/MS based full length protein sequencing. Protein samples were unspecific hydrolyzed by three different methods(proteinase K, papain and microwave-assisted acid hydrolysis) to improve overlapping degree of peptides. After LC-MS/MS analysis, peptide sequences were ...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Equus caballus (Horse) 
2022-02-15 | PXD030203 | Pride
Unspecific hydrolysis Protein sequencing De novo Maah Lc-ms/ms 
ProteomicsMetal chelating properties of fractionated potato protein hydrolysates
Metal-catalyzed lipid oxidation is a major factor in food waste, as it reduces shelf life. Addressing this issue, our study investigates the potential of hydrolysates derived from potato protein, a by-product of potato starch production, as metal chelating antioxidants. Through sequential en-zymatic...
ORGANISM(S): Solanum tuberosum (Potato) 
2024-06-23 | PXD050312 | Pride
Potato Anoxpepred Spr Sequential hydrolysis Antioxidant activity Hydrolysate 
ProteomicsPotato protein hydrolysate LC-MS/MS
Peptides and protein hydrolysates are promising alternatives to substitute chemical additives as functional food ingredients. In this study, we present a novel approach for producing a potato protein hydrolysate with improved emulsifying and foaming properties by data-driven, targeted hydrolysis. Ba...
ORGANISM(S): Solanum tuberosum (Potato) 
2024-03-04 | PXD034139 | Pride
Emulsifier peptides Potato protein Hydrolysate 
ProteomicsMyelin basic protein fragmentation by engineered human proteasomes with different catalytic phenotypes revealed direct peptide ligands of MS-associated and protective HLA class I molecules
Proteasomes exist in mammalian cells in multiple combinatorial variants due to the broadly diversified regulatory particles and catalytic subunits exchange. Here, using biotin carboxyl carrier domain of transcarboxylase from Propionibacterium shermanii fused with different proteasome subunits of cat...
ORGANISM(S): Homo sapiens (Human) 
2023-01-13 | PXD038387 | Pride
Human Lc-msms Mbp Proteosome 
UnknownEvaluation of the site-unspecified peptide identification method for proteolytic peptide mapping.
Not available
S-EPMC9057140 | biostudies-literature
UnknownThe calcium channel modulator 2-APB hydrolyzes in physiological buffers and acts as an effective radical scavenger and inhibitor of the NADPH oxidase 2.
Not available
S-EPMC10009725 | biostudies-literature
UnknownEnhancing the ROS Sensitivity of a Responsive Supramolecular Hydrogel Using Peroxizyme Catalysis.
Not available
S-EPMC10336921 | biostudies-literature
ProteomicsBODIPY-Patatin assay proteomics
The potato protein patatin embeds bioactive peptides that require targeted hydrolysis to be released as promising food additives. This study presents a patatin-specific protease assay for assessing a wide range of protease activities in high-throughput format. Conjugating patatin to the amine reacti...
ORGANISM(S): Solanum tuberosum (Potato) 
2025-10-22 | PXD049376 | Pride
Potato Bodipy-fl labeling Q exactive hf Patatin Site-specific modification enrichment 
UnknownThe nuclease domain of the SPP1 packaging motor coordinates DNA cleavage and encapsidation.
Not available
S-EPMC3592435 | biostudies-literature
UnknownInspired by Sea Urchins: Warburg Effect Mediated Selectivity of Novel Synthetic Non-Glycoside 1,4-Naphthoquinone-6S-Glucose Conjugates in Prostate Cancer.
Not available
S-EPMC7281150 | biostudies-literature
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