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UnknownIntegrated metabolic modelling reveals cell-type specific epigenetic control points of the macrophage metabolic network.
Not available
S-EPMC4617894 | biostudies-literature
no omics definedTranscription profiling of high quality RNA samples from human breast adenocarcinoma MCF-7 cells and human skeletal muscle to evalutate the experimental performance of Actichip microarrays
To evaluate the experimental performance of Actichip microarrays, a series of 10 repeated hybridisation experiments, including 5 dye swaps, were carried out with optimised target labelling, hybridisation, scanning and data analysis protocols. All the procedures were standardised to limit the impact ...
ORGANISM(S): Homo sapiens 
2007-08-01 | E-MEXP-965 | biostudies-arrayexpress
no omics definedPlatform comparison and transcription profiling using high quality RNA samples purified from human breast adenocarcinoma MCF-7 cells and human skeletal muscle on the Actichip, Affymetrix U1331 2.0 and an academic 70mer oligochip to validate the Actichip
To validate Actichip, we compared this microarray with two other well-established oligonucleotide-based platforms. We used 25-mer oligonucleotide commercial chips (Affymetrix, human genome U133A 2.0) and academic arrays prepared with a 70-mer oligonucleotide set (Operon, human whole genome version 2...
ORGANISM(S): Homo sapiens 
2007-06-01 | E-MEXP-968 | biostudies-arrayexpress
TranscriptomicsIsocitrate dehydrogenase 1 mutations prime the all-trans-retinoic acid myeloid differentiation pathway in acute myeloid leukemia HL-60 cells
Human acute myeloid leukemia HL60 cells were transduced with IDH1-WT or IDH1-R132H, and corresponding sub-clones were generated by FACS. In a first series of experiments, cells expressing wt and mutant IDH1were treated with either 1µM all-trans retinoic acid (ATRA) or with vehicle (DMSO) for 24 hrs...
ORGANISM(S): Homo sapiens 
2017-03-01 | E-MTAB-4327 | biostudies-arrayexpress
TranscriptomicsTranscriptome profiling of human anaplastic meningioma IOMM-Lee cells following silencing of HuR (Human antigen R) gene expression by siRNA
The human anaplastic meningioma IOMM-Lee cells were cultured in Dulbecco’s modified Eagle’s medium (Life Technologies, Carlsbad, California, USA) supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 mg/ml streptomycin, at 37°C in 20% O2 (5% CO2). HuR silencing was achieved by ...
ORGANISM(S): Homo sapiens 
2017-07-01 | E-MTAB-4999 | biostudies-arrayexpress
TranscriptomicsThe angiogenic switch leads to a metabolic shift in human glioblastoma
The gene expression profiles of two groups of triplicate human glioblastoma (GBM) xenografts grown in immunodeficient rats were compared. The first group of xenografts was derived from a patient biopsy with Epidermal Growth Factor Receptor (EGFR) amplification which grows highly invasive and is ind...
ORGANISM(S): Homo sapiens 
2017-07-05 | E-MTAB-4898 | biostudies-arrayexpress
TranscriptomicsNeutrophil-derived pro-inflammatory cytokine response and degranulation in function of time and dose of LPS treatment.
The purpose of the present study was to elucidate in more details the molecular mechanisms of neutrophil-mediated inflammation. We therefore investigated the time-dependent stimulatory potential of LPS from Escherichia coli on cytokine response in neutrophil-like HL-60 cells.

To get a genera...
ORGANISM(S): Homo sapiens 
2014-12-17 | E-MTAB-1393 | biostudies-arrayexpress
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