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GenomicsCyclin D1 ChIP-seq in H9 undifferentiated human pluripotent stem cells
H9 human pluripotent stem cells were grown in chemically defined media with Activin A and FGF2. Four 10cm plates with 70% confluency were used per sample. Cells were cross-linked by adding a final concentration of 1% PFA into culture media and incubated for 10 min at RT for Cyclin D1 ChIP. ChIP-seq ...
ORGANISM(S): Homo sapiens 
2016-02-01 | E-MTAB-3807 | biostudies-arrayexpress
TranscriptomicsRNA-seq of WTAP knockdown human pluripotent stem cells during the exit from pluripotency induced by Activin/Nodal inhibition
This experiment was designed to monitor the gene expression changes induced by the knockdown of WTAP in human pluripotent stem cells (hPSCs) during the exit from pluripotency induced by the inhibition of Activin/Nodal signalling. hPSCs carrying a tetracycline-inducible short hairpin RNA (shRNA) agai...
ORGANISM(S): Homo sapiens 
2018-01-04 | E-MTAB-5230 | biostudies-arrayexpress
TranscriptomicsRNA-seq of hepatocytes obtained through step-wise differentiation of hIPSCs from a patient with A1AT deficiency and its point mutation-corrected isogenic hIPSC line. Comparison to primary hepatocytes from a healthy donor and an A1AT-deficient patient.
A1AT deficiency is an autosomal not recessive disorder caused by mutations in the SERPINA1 gene. Individuals with the Z variant retain polymerised protein in the endoplasmic reticulum of hepatocytes, predisposing them to liver disease. This study primarily aimed to uncover the molecular mechanisms t...
ORGANISM(S): Homo sapiens 
2018-06-26 | E-MTAB-6781 | biostudies-arrayexpress
TranscriptomicsRNA-seq of human embryonic stem cells during cell cycle progression
The experiment was designed to look into transcriptional changes during cell cycle progression in human embryonic stem cells (hESCs). For this, FUCCI hESCs are sorted in Early G1 (EG1), Late G1 (LG1), and S/G2/M phases in triplicates, RNA was extracted and PolyA purified libraries were generated and...
ORGANISM(S): Homo sapiens 
2023-06-24 | E-MTAB-8347 | biostudies-arrayexpress
GenomicsChIP-seq of human embryonic stem cells during cell cycle progression
The experiment was designed to look into changes in key transcription factors (TFs) binding sites during cell cycle progression in human embryonic stem cells (hESCs). For this, 1 million FUCCI hESCs are sorted in Early G1 (EG1), Late G1 (LG1), and S/G2/M phases in duplicates for each experiment, and...
ORGANISM(S): Homo sapiens 
2023-06-24 | E-MTAB-8358 | biostudies-arrayexpress
TranscriptomicsATAC-seq of human embryonic stem cells during cell cycle progression
The experiment was designed to look into chromatin accessibility changes during cell cycle progression in human embryonic stem cells (hESCs). For this, FUCCI hESCs are sorted in Early G1 (EG1), Late G1 (LG1), G1/S transition and S/G2/M phases in duplicates. 100,000 were used per sample, as described...
ORGANISM(S): Homo sapiens 
2023-06-24 | E-MTAB-8348 | biostudies-arrayexpress
GenomicsActivin/Nodal signalling controls divergent transcriptional networks in pluripotent and endoderm progenitors.
Activin/Nodal signalling is necessary to maintain pluripotency of human Embryonic Stem Cells (hESCs) and to induce their differentiation towards endoderm. However, the mechanisms by which Activin/Nodal signalling achieves these opposite functions remain unclear. To unravel these mechanisms, we exami...
ORGANISM(S): Homo sapiens 
2011-11-11 | E-GEOD-19461 | biostudies-arrayexpress
ProteomicsSMAD2/3 interactome in human pluripotent stem cells
This experiment was designed to determine the interactome of SMAD2/3 in human pluripotent stem cells (hPSCs). hPSCs were cultured in standard pluripotency-promoting conditions, or induced to differentiate towards the definitive endoderm lineage for 36h. Endogenous SMAD2/3 was immunoprecipitated from...
ORGANISM(S): Homo sapiens (Human) 
2018-01-04 | PXD005285 | Pride
Smad2/3 Human pluripotent stem cells 
TranscriptomicsVallier 3day endoderm
Transcriptional profiling of H9 human embryonic stem cells differentiated towards early endoderm over 72 hours in chemically defined media.
ORGANISM(S): Homo sapiens 
2011-01-26 | E-MTAB-467 | biostudies-arrayexpress
TranscriptomicsRNA-seq of definitive endoderm and pancreatic progenitor cells derived in vitro from human pluripotent stem cells in order to study the role of transcription factor GATA6 in human pancreas development
Mutant GATA6 hPSCs were obtained by TALEN genome editing or re-programmed from patient fibroblasts. Along with wild-type H9 cells, these GATA6 mutant cell lines were differentiated into SOX17+/CXCR4+ endodermal cells (day 3) and PDX1+ pancreatic progenitor cells (day 12). The purpose of this work wa...
ORGANISM(S): Homo sapiens 
2019-08-10 | E-MTAB-5958 | biostudies-arrayexpress
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