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ProteomicsSMAD2/3 interactome in human pluripotent stem cells
This experiment was designed to determine the interactome of SMAD2/3 in human pluripotent stem cells (hPSCs). hPSCs were cultured in standard pluripotency-promoting conditions, or induced to differentiate towards the definitive endoderm lineage for 36h. Endogenous SMAD2/3 was immunoprecipitated from...
ORGANISM(S): Homo sapiens (Human) 
2018-01-04 | PXD005285 | Pride
Smad2/3 Human pluripotent stem cells 
TranscriptomicsTGF-beta/Smad2/3 signaling directly regulates several miRNAs in mouse ES Cells and early embryos
Purpose: We aimed to identify miRNAs which are induced by the Activin/Nodal effectors, P-Smad2/3, in order to further our understanding of how P-Smad2/3 controls downstream gene expression in mouse ES cells to regulate crucial biological processes. Methods: We used a previously developed Tetracyclin...
ORGANISM(S): Mus musculus 
2013-02-11 | E-GEOD-39994 | biostudies-arrayexpress
TranscriptomicsExpression data of the human hepatoblastoma cell line HepG2 treated with TGF-beta
Smad2/3 are transcription factors that engage in TGF-beta-induced transcription. We determined and analyzed HepG2 and Hep3B-specific Smad2/3 binding sites by ChIP-chip. We used expression microarrays to compare the Smad2/3 and HNF4alpha binding sites identified by ChIP-chip or ChIP-seq, respectively...
ORGANISM(S): Homo sapiens 
2011-06-08 | E-GEOD-28590 | biostudies-arrayexpress
GenomicsTranscriptional profiling of microglia lacking Smad2/3
Transcriptional profiling of microglia lacking Smad2/3
PRJNA714374 | ENA
GenomicsGene expression profiling of Smad2/3 cKO mice
Gene expression profiling of Smad2/3 cKO mice
PRJNA448677 | ENA
GenomicsSmad2/3 binding regions in NMuMG cells
Smad2/3 binding regions in NMuMG cells
PRJNA496488 | ENA
GenomicsActivin/Nodal signalling controls divergent transcriptional networks in pluripotent and endoderm progenitors.
Activin/Nodal signalling is necessary to maintain pluripotency of human Embryonic Stem Cells (hESCs) and to induce their differentiation towards endoderm. However, the mechanisms by which Activin/Nodal signalling achieves these opposite functions remain unclear. To unravel these mechanisms, we exami...
ORGANISM(S): Homo sapiens 
2011-11-11 | E-GEOD-19461 | biostudies-arrayexpress
ProteomicsElucidating the Roles of TTP and SMAD2/3 in HEK293T through LC-MS Analysis
To further investigate the mechanism underlying the transcriptional regulation of m6A modification associated genes by TTP. we performed immunoprecipitation using anti-FLAG antibody in HEK293T cells transfected with pHAGE-TTP plasmids (overexpression of TTP) and then used mass spectrometry to identi...
ORGANISM(S): Homo sapiens (Human) 
2026-05-14 | PXD056357 | Pride
Ttp Lc-ms Smad2/3 Hek293t 
ProteomicsThe phosphorylation of the Smad2/3 linker region by Nemo-like kinase regulates TGF-β signaling
Smad2 and Smad3 (Smad2/3) primarily mediates the transforming growth factor-β (TGF-β) signaling that drives cell proliferation, differentiation, and migration. The dynamics of the Smad2/3 phosphorylation provides the key mechanism for regulating the TGF-β signaling pathway. Here we identified NLK as...
ORGANISM(S): Homo sapiens (Human) 
2021-09-10 | PXD023957 | Pride
Smad3 Phosphorylation Smad2 Nlk 
TranscriptomicsTranscription profiling of mouse ES cells treated by varying levels of activated Smad2/3 over time in the presence of protein synthesis
The tet-on system was activated by treatment of the cells with doxycycline (Dox) in concentrations 1 microgram per milliliter. The Smad2/3 phosphorylation was inhibited by treatment of the cells with SB-431542. The TAG1 cells were treated with SB inhibitor and Dox activator and samples were analyzed...
ORGANISM(S): Mus musculus 
2009-01-01 | E-TABM-574 | biostudies-arrayexpress
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