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ProteomicsAssessing target engagment using proteome-wide solvent shift assays
Recent advances in mass spectrometry (MS) have enabled quantitative proteomics to become a powerful tool in the field of drug discovery, especially when applied toward proteome-wide target engagement studies. Similar to temperature gradients, increasing concentrations of organic solvents stimulate u...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026297 | Pride
Target enagagement 
ProteomicsPhosphate Starvation Signaling Increases Mitochondrial Membrane Potential through Respiration-independent Mechanisms
The mitochondrial membrane potential directly powers many critical functions of mitochondria, including ATP production, mitochondrial protein import, and metabolite transport. Its loss is a cardinal feature of aging and mitochondrial diseases, and cells closely monitor membrane potential as an indic...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-02-15 | PXD037405 | Pride
Mitochondria Phosphate 
ProteomicsLarge-scale characterization of drug mechanism of action using proteome-wide thermal shift assays - Revisions
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to characterize compound-dependent changes in thermal stability, the proteome-wide thermal shift ass...
ORGANISM(S): Homo sapiens (Human) 
2025-02-13 | PXD053138 | Pride
Thermal shift assay Proteomics Drug discovery 
ProteomicsLarge-scale characterization of drug-induced changes in thermal stability using proteome-wide thermal shift assays
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to characterize compound-dependent changes in thermal stability, the proteome-wide thermal shift ass...
ORGANISM(S): Homo sapiens (Human) 
2025-02-13 | PXD048009 | Pride
Mechanism of action Drug development 
ProteomicsAmelioration of Pathologic -Synuclein-Induced Parkinson’s Disease by Irisin
Physical activity is thought to provide clinical benefit in Parkinson’s diseas (PD). Irisin is a blood-brain barrier permeable exercise-induced polypeptide secreted by muscle that mediates, in part, the beneficial effects of exercise. Here we show that irisin prevents pathologic -synuclein (-syn) in...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-15 | PXD032670 | Pride
Irisin Alpha-synuclein Neurodegeneration Parkinson’s disease 
ProteomicsExercise Training Remodels Inguinal White Adipose Tissue Through Adaptations in Innervation, Vascularization and the Extracellular Matrix
Inguinal white adipose tissue (iWAT) is essential for the beneficial effects of exercise training on metabolic health. The underlying mechanisms for these effects are not fully understood and here, we test the hypothesis that exercise training results in a more favorable iWAT structural phenot...
ORGANISM(S): Mus musculus (Mouse) 
2023-10-24 | PXD040894 | Pride
Cold exposure Exercise training Sedentary 
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