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TranscriptomicsHigh Resolution Mapping of Mouse Embryonic Genome Activation using SMART-seq2
Single-embryo SMART-seq2 of mouse 2-cell stage embryos. The embryos have been generated with in vitro fertilization (IVF) and are staged as hours post-IVF.
ORGANISM(S): Mus musculus 
2026-06-24 | E-MTAB-15452 | biostudies-arrayexpress
TranscriptomicsTime course of Embryonic Genome Activation Following Kdm5b Overexpression using SMART-seq2
RNA-seq of single mouse embryos using SMART-seq2. The embryos have been generated with IVF and are staged as hours post-IVF. At the zygote stage (~ 4h post-IVF), the embryos have been microinjected with 1.7 μg/μL Kdm5b wild-type (WT) or catalytic mutant (CM) mRNA. Non-injected embryos are denoted as...
ORGANISM(S): Mus musculus 
2026-06-24 | E-MTAB-15454 | biostudies-arrayexpress
TranscriptomicsSMART-seq2 of Kdm5b Overexpression in Fully Grown Oocytes
RNA-seq of single Fully Grown Oocytes using SMART-seq2. The oocytes were collected from female FVB mice, approximately. 44-48 h post-PMSG injection. The oocytes were microinjected with 1.3-1.7 μg/μL Kdm5b wild-type (WT) or catalytic mutant (CM) mRNA. Noninjected oocytes are denoted as “Noninj”. The ...
ORGANISM(S): Mus musculus 
2026-06-24 | E-MTAB-15453 | biostudies-arrayexpress
TranscriptomicsHigh Resolution Mapping of Mouse Embryonic Genome Activation using SMART-seq2 with in vitro polyadenylation
Single-embryo SMART-seq of mouse Embryonic Genome Activation with 2-cell stage embryos collected after 2 h precision in vitro fertilization (IVF) at the following timepoints: 17, 20, 23, and 26 h post-IVF. Individual embryos were lysed in total cell lysis buffer, and the lysates were split to perfor...
ORGANISM(S): Mus musculus 
2026-06-24 | E-MTAB-16935 | biostudies-arrayexpress
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