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ProteomicsIn-Gel digest and filter-aided sample preparation to identify Dermatophagoides - and yeast protein antigens in severe equine asthma.
Severe equine asthma (SEA) is a common, chronic respiratory disease of horses characterized by hyperreactivity to hay dust which has many similarities to severe neutrophilic asthma in humans. A 2D immunoblot approach was used to identify quantitative differences in the recognition of the two-dimensi...
ORGANISM(S): Dermatophagoides Pteronyssinus Saccharomyces Cerevisiae 
2024-01-26 | PXD045568 | panorama
ProteomicsAnalysis of N-Glycosylation for SARS-CoV-2 expressed receptor-binding domain VOCs and membrane protein with LC-MS.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect human cells by first attaching to the ACE-2 receptor via its receptor-binding domain (RBD) in the spike protein. We investigated the influence of N-glycosylation sites of the RBD and the membrane (M) protein on IgG antibody bind...
ORGANISM(S): Severe Acute Respiratory Syndrome Coronavirus 2 
PXD048930 | panorama
ProteomicsIn-Gel Digest to identify Aspergillus fumigatus protein antigens in severe equine asthma (SEA)
Aspergillus fumigatus (Asp f) has been described as a major extrinsic stimulus of SEA and is a common mold species in hay. Aiming to identify disease-relevant antigens, we analyzed Asp f as a source, in an immuno-proteomics approach on two-dimensional immunoblots. For 21 of 289 spots serum Ig bindin...
ORGANISM(S): Aspergillus Fumigatus 
PXD045869 | panorama
ProteomicsImproving peptide fragment ion detection using travelling wave ion mobility spectrometry with signal enhancement MSE (seMSE)
Recently, a solution to inherent poor ion beam sampling of time-of-flight (TOF) mass analyzers was proposed by restricting detection to ions expected at specific arrival times after being separated in a travelling wave ion mobility spectrometry (TWIMS) cell. Here, we show that this approach can be a...
ORGANISM(S): Oryctolagus Cuniculus Escherichia Coli Bos Taurus Saccharomyces Cerevisiae 
PXD030431 | panorama
ProteomicsAntimicrobial peptides of the apidaecin family inhibit the bacterial ribosome by multimodal mechanisms involving three different sites on the large ribosomal subunit
Proline-rich antimicrobial peptides (PrAMPs) inhibit bacterial ribosomes by binding to the polypeptide exit tunnel (PET) near the peptidyl transferase center. Api137, an optimized derivative of honeybee apidaecin, traps the release factor (RF) at the ribosome, thereby arresting the ribosomes at stop...
ORGANISM(S): Escherichia Coli 
PXD044892 | panorama
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