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ProteomicsModulating the selectivity of affinity absorbents to multi-phosphopeptides by a novel competitive substitution strategy
Although many affinity adsorbents have been developed for phosphopeptides enrichment, high-specifically capturing the multi-phosphopeptides is still a big challenge. Here, we investigated the mechanism of phosphate ions coordination and substitution on affinity adsorbents surfaces and modulated the ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080735 | MassIVE
ProteomicsIntegrated Protein Precipitation–SPE Workflow for Deep Top-Down Proteomic Analysis of Human Serum
Top-down proteomics of human serum is hindered by extreme dynamic range and cumbersome prefractionation. Here we establish a fully solution-based protein precipitation–solid-phase extraction (PP–SPE) workflow for deep, low-molecular-weight-biased proteoform profiling. Using systematically optimized ...
ORGANISM(S): Homo Sapiens 
PXD073228 |
ProteomicsGlobal quantification of intact proteins via chemical isotope labeling and mass spectrometry
Although thousands of intact proteins can be feasibly identified in recent years, global quantification of intact proteins is still challenging. Herein, we develop a high-throughput strategy for global intact protein quantification based on chemical isotope labeling. The isotope incorporation effici...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-04-30 | PXD012526 | Pride
Intact protein quantification Ptop Chemical isotope labeling Highest isotopic peak Mass spectrometry 
ProteomicsBSA,HSA,myoglobin,mouse liver LC-MS/MS
We systematically explored the VUV photochemical reactions of aqueous halides Cl−, Br−, and I− with native proteins and peptides under the irradiation of a 10-ns single pulse of 193-nm ArF VUV laser
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Mus musculus (Mouse) Equus caballus (Horse) 
2022-08-15 | PXD024795 | Pride
Bsa Hsa Mouse liver lc-ms/ms Myoglobin 
ProteomicsDetecting Proteins Glycosylation by a Homogeneous Reac-tion System with Zwitterionic Gold Nanoclusters
Homogeneous gold nanoclusters (Au NCs) have been widely utilized in drug delivery, chemical sensing, bioassays and bio-labeling due to their unique physical and chemical properties. However, few attentions have been paid on their application in detecting protein post-translational modifications. Her...
ORGANISM(S): Mus musculus (Mouse) 
2017-03-29 | PXD005635 | Pride
Gold cluster Glycopeptides Homogeneous reaction system 
ProteomicsSpecific mixing facilitates the comparative quantification of phosphorylation sites with significant dysregulations
It is still a big challenge to accurately quantify the proteins or proteins PTM sites with extreme relative abundances in comparative protein samples, such as the significantly dysregulated ones. Herein, a novel quantification strategy, Mixing at Specific Ratio (MaSR) before isotope labeling, had be...
ORGANISM(S): Candida albicans (Yeast) Homo sapiens (Human) Escherichia coli 
2016-12-06 | PXD005181 | Pride
Lc-msms Mixing at specific ratio Comparative phosphoproteome quantification Stable isotope labeling Extreme relative abundance 
ProteomicsModulating the selectivity of affinity absorbents to multi-phosphopeptides by a novel competitive substitution strategy
Although many affinity adsorbents have been developed for phosphopeptides enrichment, high-specifically capturing the multi-phosphopeptides is still a big challenge. Here, we investigated the mechanism of phosphate ions coordination and substitution on affinity adsorbents surfaces and modulated the ...
ORGANISM(S): Homo sapiens (Human) 
2016-08-03 | PXD004252 | Pride
Mass spectrometry Multi-phosphopeptide enrichment Pre-coordination Phosphoproteomics Competitive substitution 
ProteomicsElectrospray ionization in concentrated acetonitrile vapor improves the performance of mass spectrometry for proteomic analyses
Suppressing the background interferences and enhancing the analyte signals are long-term goals in high performance electrospray ionization mass spectrometry (ESI-MS) analyses. We observed that performing electrospray in the presence of a concentrated acetonitrile atmosphere suppresses background int...
ORGANISM(S): Homo sapiens (Human) 
2017-01-04 | PXD005132 | Pride
Background interference Mass spectrometry Electrospray ionization Acetonitrile 
MetabolomicsMulti-hierarchical profiling the structure-activity relationships of engineered nanomaterials at nano-bio interfaces
Increasing concerns over the possible risks of nanotechnology necessitates breakthroughs in structure-activity relationship (SAR) analyses of engineered nanomaterials (ENMs) at nano-bio interfaces. However, current nano-SARs are often based on univariate assessments and fail to provide tiered views ...
2020-01-23 | MTBLS721 | MetaboLights
UnknownEf-cd locus shortens rice maturity duration without yield penalty.
Not available
S-EPMC6744900 | biostudies-literature
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