The tobacco hornworm, Manduca sexta, is a lepidopteran model species widely used to study insect biochemical processes. While some of its larval hemolymph proteins are well understood, a detailed proteomic analysis was unavailable until 2016, revealing features such as correlation with transcriptome...
To examine whether P26 is involved in the extracellular melanization pathway, we used HearNPV and cotton bollworm as a testing system and detected the presence of HearNPV P26 in hemolymph from the virus-infected insect via liquid chromatography tandem mass spectrometry analysis. P26 was one of the m...
The promiscuous biotin ligase TurboID was fused to Tat for precise and temporal biotinylation of surrounding proteins, which could be enriched by streptavidin and identified by mass spectrometry.
This project identifies GALNT1-interacting proteins in primary fibroblast-like synoviocytes (RASFs) derived from rheumatoid arthritis patients using co-immunoprecipitation (co-IP) coupled with mass spectrometry (MS). Briefly, RASF lysates were incubated with anti-GALNT1 antibody to capture GALNT1-a...
