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ProteomicsProteome and phosphoproteome analyses reveal the kinase regulatory network involved in glycogen synthesis kinase 3β
To analyze the roles of GSK-3β in podocytes, GSK-3β knockdown lentivirus by Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas)9 was applied to establish stable cell lines. Phosphoproteome and proteome evaluation was conducted using TMT labeled LC-MS/MS...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-10 | PXD024543 | Pride
Gsk-3β Proteome Podocytes Diabetic nephropathy Phosphoproteome 
ProteomicsIntegrated Optoproteomics and AP-MS Reveals interactome Remodeling Upon NLRP3 Inflammasome Activation
NOD-like receptor family pyrin domain-containing 3 (NLRP3) is a critical intracellular danger sensor that forms inflammasome complexes to drive inflammatory caspase activation; however, the components and dynamic organization of the NLRP3 inflammasome remain incompletely defined. Here, we systematic...
ORGANISM(S): Mus Musculus 
2026-06-11 | PXD079555 |
ProteomicsA C-terminal tail deletion of histidine kinase WalK/VicK disrupted transcriptional regulation in Streptococcus mutans
An essential two-component system WalRK is required for biofilm formation of streptococcus mutans mainly through transcriptional regulations.
ORGANISM(S): Streptococcus Mutans Ua159 
2021-09-30 | PXD028893 |
ProteomicsSimultaneous profiling of phosphorylation and S-nitrosylation during maize seed germination
We developed a phosphate-affinity tag-switch (PAT-switch) proteomics workflow enabling simultaneous, site-specific profiling of phosphorylation and S-nitrosylation during maize seed germination.
ORGANISM(S): Zea Mays 
2026-01-21 | PXD073327 |
ProteomicsTandem proteomic and N-glycoproteomic analysis of extracellular vesicles
Extracellular vesicles (EVs) derived from diverse biofluids have emerged as promising noninvasive diagnostic biomarkers or signatures for various diseases. EV-associated N-glycoproteins are crucial for the biological functions of EVs and hold significant potential for disease diagnosis and prognosis...
ORGANISM(S): Homo Sapiens 
2024-11-05 | PXD057512 |
Proteomicsproteomic and N-glycoproteomic analysis of extracellular vesicles for biofluids
Extracellular vesicles (EVs) derived from diverse biofluids have emerged as promising noninvasive diagnostic biomarkers or signatures for various diseases. EV-associated N-glycoproteins are crucial for the biological functions of EVs and hold significant potential for disease diagnosis and prognosis...
ORGANISM(S): Homo Sapiens 
2024-11-05 | PXD057515 |
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