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MetabolomicsAirway Dysbiosis Accelerates Lung Function Decline in Chronic Obstructive Pulmonary Disease (Human UPLC-MS/MS assays)

Progressive decline in lung function is a hallmark of chronic obstructive pulmonary disease (COPD). Although airway dysbiosis occurs in COPD, whether it contributes to disease progression remains unknown. Here, through a longitudinal analysis on 181 COPD individuals from two large cohorts involvi...

2023-06-12 | MTBLS5423 | MetaboLights
MetabolomicsAirway Dysbiosis Accelerates Lung Function Decline in Chronic Obstructive Pulmonary Disease (Murine UPLC-MS/MS assays)

Progressive lung function decline is a hallmark of chronic obstructive pulmonary disease (COPD). Airway dysbiosis occurs in COPD, but whether it contributes to disease progression remains unknown. Here we showed, through a longitudinal analysis on two cohorts involving four UK centers, that basel...

2023-06-12 | MTBLS6894 | MetaboLights
ProteomicsOGA-mediated protein glycosylation regulates pluripotency states in human embryonic stem cells
In our previous study, we uncovered that OGA significantly elevated in naïve hESC. Therefore, we sought to investigate the effects of O-GlcNAc on hESC and the interconvert of both pluripotent states. Depletion of OGA, which results in global O-GlcNAcylation increased, would impair naïve hESC pluripo...
ORGANISM(S): Homo sapiens (Human) 
2024-08-10 | PXD041605 | Pride
Human embryonic stem cells，oga，o-glcnac 
ProteomicsA Rapid and Sensitive Single-cell Proteomic Method Based on Fast LC Separation, Deep-learning RT Prediction and MS1-only Acquisition
We report an integrate strategy that combines fast liquid chromatography (LC) separation, deep learning-based retention time (RT) prediction and MS1-only acquisition for sensitive and rapid single-cell proteome analysis. Finally, SCP-MS1 was applied to sorafenib resistant HepG2 cells.
ORGANISM(S): Homo Sapiens 
2022-08-26 | PXD036211 |
ProteomicsCell Data-Indenpendent acquisition LC-MS/MS
In this work, a microwell-chip was prepared and modified. The microwell-chip was used for extraction of metabolites and subsequent protein digestion. Next, direct electrospray ionization mass spectrometry (ESI-MS) was adopted for metabolome identification and a data independent acquisition (DIA)-MS ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-11-08 | PXD025634 | Pride
Dia Cell Lc-ms/ms 
ProteomicsIdentification of N-Glycopeptides based on Glycocarrier Strategy
In this work, we developed a glycocarrier strategy to amplify MS signals with isobaric labeling for highly sensitive intact N-glycopeptide characterization from single and small numbers of cells without enrichment. Successful application in cell lines and microglia from the regional mouse brain demo...
ORGANISM(S): Homo Sapiens Mus Musculus 
2022-11-18 | PXD038212 |
ProteomicsTMT proteomics analysis of cerebrospinal fluid from patients with cerebral venous sinus thrombosis
In the present study, we detected the alterations of the CSF protein spectrum of CVST patients by TMT technique, screen differentially expressed proteins, analyze the functions of these signals through bioinformatics methods, and finally validate the key molecules through parallel reaction monitorin...
ORGANISM(S): Homo Sapiens 
2022-11-22 | PXD038259 |
ProteomicsProteomic analysis of TMT of sea urchin(Strongylocentrotus intermedius) under high temperature stress
In order to investigate the protein expression of S. intermedius under high temperature stress. The study was conducted with the high-temperature resistant strain of S. intermedius and the control group of S. intermedius.
ORGANISM(S): Strongylocentrotus Intermedius 
2023-07-18 | PXD043884 |
ProteomicsThe TET-Sall4-BMP regulatory axis controls craniofacial cartilage development
Craniofacial microsomia (CFM) is a congenital defect that usually results from aberrant development of embryonic pharyngeal arches. However, the molecular basis of CFM pathogenesis is largely unknown. Here, we employ the zebrafish model to investigate mechanisms of CFM pathogenesis. In early embryos...
ORGANISM(S): Danio Rerio 
2024-02-02 | PXD049147 |
ProteomicsCircular RNA circBNC2 regulates epithelial cell G2-M arrest to prevent fibrotic maladaptive repair
Epithelial cells arrested in G2/M phase of the cell cycle after injury is a key checkpoint for determining the wound-healing to either normal cell proliferation or fibrosis. The mechanism that regulates the fibrogenic response after injury is not well understood. Here, we identified a kidney- and li...
ORGANISM(S): Homo Sapiens 
2022-04-18 | PXD033284 |
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