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TranscriptomicsEssential role of lncRNA binding for WDR5 maintenance of active chromatin and embryonic stem cell pluripotency
Here we delineate the roles of RNA binding for WDR5 function on chromatin state and murine embryonic stem cell (ESC) maintenance. Examination of global transcriptional changes and mapping genome-wide transcription factors occupancy at distinct time points during the transdifferentiation process
ORGANISM(S): Mus musculus 
2014-02-12 | E-GEOD-53035 | biostudies-arrayexpress
GenomicsInteraction with WDR5 promotes target gene recognition and tumorigenesis by MYC
We report a comparison of the genome-wide binding patterns of MYC and WDR5, and the effects of a mutation in MYC (WBM) that disrupt the MYC-WDR5 interaction. The experimental design included two distinct experimental strategies. For comparison of genome-wide binding patters of wild-type (WT) and WDR...
ORGANISM(S): Homo sapiens 
2015-03-30 | E-GEOD-60897 | biostudies-arrayexpress
GenomicsWdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network
The embryonic stem (ES) cell transcriptional and epigenetic networks are critical for the maintenance of ES cell self-renewal. However, it remains unclear whether components of these networks functionally interact and if so, what factors mediate such interactions. Here we show that WD-repeat protein...
ORGANISM(S): Mus musculus 
2011-04-08 | E-GEOD-22934 | biostudies-arrayexpress
TranscriptomicsEffects of KAT2B and WDR5 depletion on hepatocyte gene expression
During fasting, increases in circulating pancreatic glucagon maintain glucose balance by up-regulating hepatic gluconeogenesis. Triggering of the cAMP pathway stimulates the gluconeogenic program through the phosphorylation of CREB and via the de-phosphorylation of the CREB coactivator CRTC2. Hormon...
ORGANISM(S): Mus musculus 
2014-04-21 | E-GEOD-47179 | biostudies-arrayexpress
ProteomicsWDR5 protein interactions at the WIN-site
The WIN site of WDR5 is a druggable pocket that impairs WDR5 protein function and carries therapeutic potential for treating cancer. This study evaluates the protein interactions affected by small molecule blockade of this surface on WDR5. Inhibited and uninhibited WDR5-containing complexes from HEK...
ORGANISM(S): Homo sapiens (Human) 
2021-01-25 | PXD019209 | Pride
Silac Wdr5 Immunoprecipitation Signaling Chromatin 
GenomicsA Role for WDR5 in Integrating Threonine 11 Phosphorylation to Lysine 4 Methylationon Histone H3 and in Prostate Cancer
Upon androgen stimulation, PKN1-mediated histone H3 threonine 11 phosphorylation (H3T11P) promotes AR target genes activation. However, the underlying mechanism is not completely understood. Here, we show that WDR5, a subunit of the SET1/MLL complex, interacts with H3T11P and this interaction facili...
ORGANISM(S): Homo sapiens 
2014-07-01 | E-GEOD-55279 | biostudies-arrayexpress
TranscriptomicsThe histone H3 lysine 4 presenter WDR5 induces N-Myc target gene transcription and promotes neuroblastoma [gene expression]
WDR5 is an important co-factor for N-Myc-regulated transcriptional activation and tumorigenesis We analysed whether WDR5 siRNAs modulate gene expression Neuroblastoma BE(2)-C cells were treated with control siRNA, WDR5 siRNA-1, and WDR5 siRNA-2 for 40 hours, and subjected to differential gene expres...
ORGANISM(S): Homo sapiens 
2015-12-31 | E-GEOD-69861 | biostudies-arrayexpress
GenomicsGenome-wide binding of WDR5 and GRHL3
The antagonistic actions of Polycomb and Trithorax are responsible for proper cell fate determination in mammalian tissues. In the epidermis, a self-renewing epithelium, previous work has shown that release from Polycomb repression only partially explains differentiation gene activation. We now show...
ORGANISM(S): Homo sapiens 
2012-11-10 | E-GEOD-42180 | biostudies-arrayexpress
GenomicsThe histone H3 lysine 4 presenter WDR5 induces N-Myc target gene transcription and promotes neuroblastoma [ChIP-Seq]
WDR5 is an important co-factor for N-Myc-regulated transcriptional activation and tumorigenesis Using ChIP-Seq, We profiled key epigenetic marks H3K4 trimethylation in BE(2)-C neuroblastoma cells transfected with control siRNA or WDR5 siRNA-1 at N-Myc target gene promoters The results showed knockdo...
ORGANISM(S): Homo sapiens 
2015-12-31 | E-GEOD-70098 | biostudies-arrayexpress
GenomicsChIP seq of SiHa RhoC overexpressing cells with RhoC and WDR5 antibodies
The aim of the experiment was to identify regions of DNA bound by RhoC and WDR5. SiHa cells overexpressing RhoC were crosslinked, digested with MNase and sonicated. The fragmented DNA that bound to RhoC and WDR5 was pulled down using RhoC and WDR5 antibodies and sequenced using the Illumina HiSeq pl...
ORGANISM(S): Homo sapiens 
2022-12-31 | E-MTAB-12164 | biostudies-arrayexpress
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