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ProteomicsSimultaneous N-deglycosylation and digestion of human and chicken proteins on S-Traps
N-linked glycosylation is an important post-translational modification that is difficult to identify and quantify in traditional bottom-up proteomics experiments. Enzymatic deglycosylation of proteins by peptide:N-glycosidase F (PNGase F) prior to digestion and subsequent mass spectrometry analysis ...
ORGANISM(S): Homo sapiens (Human) Gallus gallus (Chicken) 
2023-02-09 | PXD037921 | Pride
Human Deglycosylation Chicken S-trap Lc-ms/ms 
ProteomicsInfluence of generational obesity on the visceral adipose proteome
Obesity is a global health concern affecting over 650 million adults with a major contributor being an increased consumption of a high-fat diet. Maternal obesity often results in an increased risk of offspring developing obesity. In this study, we examined the effect of diet on visceral adipose in ...
ORGANISM(S): Mus musculus (Mouse) 
2024-10-17 | PXD042092 | Pride
Proteomics Suspension trapping Generational obesity Omental tissue Adipose tissue Mouse Diet S-trap 
UnknownStructural insights into S-lignin O-demethylation via a rare class of heme peroxygenase enzymes.
Not available
S-EPMC11842817 | biostudies-literature
UnknownSingle-step Replacement of an Unreactive C-H Bond by a C-S Bond Using Polysulfide as the Direct Sulfur Source in Anaerobic Ergothioneine Biosynthesis.
Not available
S-EPMC8302205 | biostudies-literature
TranscriptomicsExpression data of germinating Arabidopsis seeds
In depth temporal profiling of transcript changes at 10 time points during germination in Arabidopsis seed was carried out. The time course utilised, encompassed seed maturation, stratification, germination and post-germination and provided a global investigation into the tightly regulated, phasic c...
ORGANISM(S): Arabidopsis thaliana 
2011-09-08 | E-GEOD-30223 | biostudies-arrayexpress
UnknownSimultaneous N-Deglycosylation and Digestion of Complex Samples on S-Traps Enables Efficient Glycosite Hypothesis Generation.
Not available
S-EPMC9893465 | biostudies-literature
ProteomicsImmunoaffinity-free chromatographic purification of ovarian cancer biomarker CA125 (MUC16) from blood serum
The present work developed a non-affinity-based chromatographic method to enrich MUC16 from serum. The enriched MUC16 sample was further processed using a Midi Top 14 abundant protein depletion column. Peptides identified using bottom-up proteomics yielded 1–8% coverage of MUC16. Additionally, MUC16...
ORGANISM(S): Homo sapiens (Human) 
2024-10-17 | PXD053289 | Pride
Human Plasma Ovarian Muc16 Ca125 
MetabolomicsLaser-assisted rapid evaporative ionisation mass spectrometry (LA-REIMS) as a metabolomics platform in cervical cancer screening

BACKGROUND: The introduction of high-risk human papillomavirus (hrHPV) testing as part of primary cervical screening is anticipated to improve sensitivity, but also the number of women who will screen positive. Reflex cytology is the preferred triage test in most settings but ha...

2022-02-15 | MTBLS1371 | MetaboLights
UnknownImplications for an imidazol-2-yl carbene intermediate in the rhodanase-catalyzed C-S bond formation reaction of anaerobic ergothioneine biosynthesis.
Not available
S-EPMC8570591 | biostudies-literature
MetabolomicsPseudomonas syringae infectivity correlates to altered transcript and metabolite levels of Arabidopsis Mediator mutants

Rapid metabolic responses to pathogens are essential for plant survival and depend on numerous transcription factors. Mediator is the major transcriptional co-regulator for integration and transmission of signals from transcriptional regulators to RNA polymerase II. Using four Arabidopsis Mediato...

2024-02-19 | MTBLS8105 | MetaboLights
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