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MetabolomicsMetabolic Profiling of Escherichia coli-Based Cell-Free Expression Systems for Process Optimization
Biotechnology has transformed the production of various chemicals and pharmaceuticals due to its efficient and selective processes, but it is inherently limited by its use of live cells as 'biocatalysts.' Cell-free expression (CFE) systems, which use a protein lysate isolated from whole cells, have ...
2020-06-02 | MTBLS1079 | MetaboLights
ProteomicsThe Whole cell lysate and Crude membrane of HEK293T cells
We perform the experiment in order to compare identified membrane proteins from whole cell lysate and membrane fraction in HEK293T cell. The isolation of membrane fractions is critical to obtain a more comprehensive membrane proteome, especially with proteins containing transmembrane domains.
ORGANISM(S): Homo Sapiens (human) 
2022-04-28 | PXD027472 | JPOST Repository
ProteomicsA Tiered Approach to Human Synapse Proteomics: Optimized LC-MS/MS Analysis of Whole-Tissue Lysate and Synaptosome Preparations from Frozen Post-Mortem Brain Samples
Recent advancements in neuroproteomics have enabled detailed analysis of protein expression and function in the human brain, improving our understanding of the relationship between genetics, cell biology of neurological and psychiatric disorders and their clinical diagnosis. Synaptic dysfunction oft...
ORGANISM(S): Homo sapiens (Human) 
2026-05-25 | PXD076278 | Pride
ProteomicsThe Whole cell lysate and Crude membrane of HEK293T cells
We perform the experiment in order to compare identified membrane proteins from whole cell lysate and membrane fraction in HEK293T cell. The isolation of membrane fractions is critical to obtain a more comprehensive membrane proteome, especially with proteins containing transmembrane domains.
ORGANISM(S): Homo Sapiens (human) 
2024-09-10 | PXD027459 | JPOST Repository
ProteomicsAnalysis of isotopically depleted E. coli MG1655 whole cell lysate
Analysis of whole cell lysate from E. coli MG1655 grown in isotopically depleted M9 minimal medium. Medium was isotopically depleted by formulation of the medium with glucose and ammonium sulfate depleted of carbon-13 and nitrogen-15, respectively. Dataset is licensed under the terms of the Creative...
ORGANISM(S): Escherichia Coli Str. K-12 Substr. Mg1655 (ncbitaxon:511145) 
2022-01-13 | MSV000088679 | MassIVE
no omics definedmRNA expression profiling of human mesenchymal stromal cell samples grown in vitro under different conditions (foetal calf serum (FCS) based medium versus human platelet lysate (PL) based medium)
Genetic profiling after stem cell culturing. Culturing cells from normal donors, grown in 2 culture media
ORGANISM(S): Homo sapiens 
2023-03-01 | E-MTAB-12272 | biostudies-arrayexpress
ProteomicsKM-12 Whole Cell Lysate Proteomics
The KM-12 cell line, representative of colorectal cancer indications, was used to explore the effect that multiple compounds would have in the profile of transcription factor occupancy. Cells were dosed with 3 concentrations of each of the 5 compounds provided for 3 different timepoints. Both a chro...
ORGANISM(S): Homo sapiens (Human) 
2026-04-06 | PXD065035 | Pride
Lc-msms Colorectal cancer Timstof 
ProteomicsComparison between matched microdissected and whole tissue breast cancer specimen
A total of 38 flash-frozen breast cancer tissues were collected from three medical centers in the Netherlands (i.e. National Cancer institute: n=19; Radboud University Medical Center: n=4; Erasmus University Medical Center: n=15). All tissues were analyzed as laser capture microdissected (LCM) and w...
ORGANISM(S): Homo sapiens (Human) 
2016-03-31 | PXD002381 | Pride
Human Estrogen receptor positive breast cancer Lcm Whole tissue 
ProteomicsALPK3 Whole Cell Lysate In vitro Kinase assay and phosphoproteomics
A whole cell lysate in vitro kinase assay using ALPK3 was performed to identify novel substrates.
ORGANISM(S): Homo sapiens (Human) 
2023-01-19 | PXD038544 | Pride
Alpk3 
UnknownAldehyde Production in Crude Lysate- and Whole Cell-Based Biotransformation Using a Noncanonical Redox Cofactor System.
Not available
S-EPMC8294662 | biostudies-literature
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