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ProteomicsBenchmarking CHIMERYS using Wide Window Acquisition and Classical Narrow Isolation Window Data Dependent Acquisition
A comprehensive proteome map is essential to elucidate molecular pathways and protein functions. Although great improvements in sample preparation, instrumentation and data analysis already yield-ed impressive results, current studies suffer from a limited proteomic depth and dynamic range there-for...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2024-02-13 | PXD045500 | Pride
E. coli Wide window acquisition Yeast Direct injection Micropillar array chromatography Vanquish neo Hela Orbitrap exploris 480 Single cells Trap-and-elute 
ProteomicsWide-Window Acquisition Improves Proteome Coverage and Measurement Throughput for Label-Free Single-Cell Proteomics
The sensitivity of single-cell proteomics (SCP) has increased dramatically in recent years due to advances in experimental design, sample preparation, separations and mass spectrometry instrumentation. However, further increasing the sensitivity of SCP methods and instrumentation will enable the stu...
ORGANISM(S): Homo sapiens (Human) 
2023-07-11 | PXD037527 | Pride
Label-free Proteome Single-cell proteomics Wide-window acquisition 
ProteomicsWide Window Acquisition and AI-based data analysis to reach deep proteome coverage for a wide sample range, including single cell proteomic inputs – Co-immunoprecipitation samples
A comprehensive proteome map is essential to elucidate molecular pathways and protein functions. Although great improvements in sample preparation, instrumentation and data analysis already yielded impressive results, current studies suffer from a limited proteomic depth and dynamic range therefore ...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-13 | PXD037985 | Pride
Mus musculus Co-immunoprecipitation Micropillar array chromatography Smarca5 Orbitrap exploris 480 Ultimate3000 rslcnano Protein-protein interactions Trap-and-elute 
UnknownPrintable, wide band-gap chalcopyrite thin films for power generating window applications.
Not available
S-EPMC3957128 | biostudies-literature
Proteomics15 cm Aurora Gen3 column benchmarking on an Exploris 480
A comprehensive proteome map is essential to elucidate molecular pathways and protein functions. Although great improvements in sample preparation, instrumentation and data analysis already yield-ed impressive results, current studies suffer from a limited proteomic depth and dynamic range there-for...
ORGANISM(S): Homo sapiens (Human) 
2024-02-13 | PXD045457 | Pride
Vanquish neo Orbitrap exploris 480 Trap-and-elute 
ProteomicsBenchmarking of 5.5 cm, 50 cm and 110 cm micropillar array columns as compared to packed bed columns
A comprehensive proteome map is essential to elucidate molecular pathways and protein functions. Although great improvements in sample preparation, instrumentation and data analysis already yield-ed impressive results, current studies suffer from a limited proteomic depth and dynamic range there-for...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2024-02-13 | PXD039576 | Pride
E. coli Yeast Direct injection Micropillar array chromatography Vanquish neo Hela Orbitrap exploris 480 Trap-and-elute 
ProteomicsDIA+: A novel data-independent acquisition method combines multiple precursor charges to boost peptide signal.
Data-independent acquisition (DIA) methods aim to expand the benefits of low-throughput targeted proteomics to proteome-wide analyses. These methods rely on the use of several broadband isolation windows that select and fragment all peptide ions within a cycle. Isolation windows differ in that (I) t...
ORGANISM(S): Homo sapiens (Human) 
2018-10-11 | PXD010210 | Pride
Hela Dia 
ProteomicsCharacterization of cerebrospinal fluid via data-independent acquisition mass spectrometry
Cerebrospinal fluid (CSF) is in direct contact with the brain and serves as a valuable specimen to examine diseases of the central nervous system through analyzing its components. These include the analysis of metabolites, cells as well as proteins. For identifying new suitable diagnostic protein bi...
ORGANISM(S): Homo sapiens (Human) 
2018-09-17 | PXD010698 | Pride
Cerebrospinal fluid Dia Proteomics Data-independent acquisition mass spectrometry Csf Spectral library 
ProteomicsCharacterization of cerebrospinal fluid via data-independent acquisition mass spectrometry
Cerebrospinal fluid (CSF) is in direct contact with the brain and serves as a valuable specimen to examine diseases of the central nervous system through analyzing its components. These include the analysis of metabolites, cells as well as proteins. For identifying new suitable diagnostic protein bi...
ORGANISM(S): Homo sapiens (Human) 
2018-09-17 | PXD010708 | Pride
Cerebrospinal fluid Dia Proteomics Data-independent acquisition mass spectrometry Csf Spectral library 
ProteomicsRepurposed 3D Printer Allows Economical and Programmable Fraction Collection for Proteomics of Nanogram Scale Samples
In this work we describe the construction and application of a repurposed 3D-printer as a fraction collector. We utilise a nanoLC to ensure minimal volumes and surfaces although any LC can be coupled. The setup operates as a high-pH fractionation system capable of effectively working with nanogram s...
ORGANISM(S): Homo sapiens (Human) 
2024-07-12 | PXD051148 | Pride
Dda dia wwa lc-ms/ms Phosphoproteome Proteomics Two-dimensional peptide separation Rna-binding protein 
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